IKZF1 sirna

IKZF1 siRNA (Human)

Cat. Num. AAA8205570

• Gene Names: IKZF1; IK1; LYF1; LyF-1; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IKZF1; IKZF1 siRNA (Human); IK1; IKAROS; LYF1; ZNFN1A1; DNA-binding protein Ikaros; Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1; IKZF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IKZF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 477

• Applicable Applications for IKZF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IKZF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IKZF1 sirna

siRNA to inhibit IKZF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 334085209
• NCBI GeneID: 10320
• NCBI Accession #: NP_001207694.1
• NCBI GenBank Nucleotide #: NM_001220765.2
• UniProt Accession #: Q13422; O00598; Q53XL2; Q69BM4; Q8WVA3; A4D260; B4E0Z1; D3DVM5
• Molecular Weight: 24,470 Da
• NCBI Official Full Name: DNA-binding protein Ikaros isoform 2
• NCBI Official Synonym Full Names: IKAROS family zinc finger 1 (Ikaros)
• NCBI Official Symbol: IKZF1
• NCBI Official Synonym Symbols: IK1; LYF1; LyF-1; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452
• NCBI Protein Information: DNA-binding protein Ikaros
• UniProt Protein Name: DNA-binding protein Ikaros
• Protein Family: DNA-binding protein
• UniProt Gene Name: IKZF1
• UniProt Synonym Gene Names: IK1; IKAROS; LYF1; ZNFN1A1
• UniProt Entry Name: IKZF1_HUMAN

NCBI Description

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]

Uniprot Description

Ikaros: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development. Deletions in Ikaros have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Seven alternatively spliced human isoforms have been described.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: centric heterochromatin; protein complex; cytoplasm; nucleus

Molecular Function: DNA binding; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding; transcription factor activity

Biological Process: Peyer's patch development; thymus development; transcription, DNA-dependent; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; cell cycle; lymph node development; positive regulation of neutrophil differentiation; natural killer cell differentiation; retina development in camera-type eye; B cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; mesoderm development; positive regulation of NK T cell differentiation; T cell differentiation