IGSF8 sirna

IGSF8 siRNA (Human)

Cat. Num. AAA8218663

• Gene Names: IGSF8; EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IGSF8; IGSF8 siRNA (Human); CD81P3; EWI2; KCT4; Immunoglobulin superfamily member 8; IgSF8; CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; EWI-2; Keratinocytes-associated transmembrane protein 4; KCT-4; LIR-D1; Prostaglandin regulatory-like protein; PGRL; CD antigen CD316; IGSF8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IGSF8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 613

• Applicable Applications for IGSF8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IGSF8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IGSF8 sirna

siRNA to inhibit IGSF8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 331284207
• NCBI GeneID: 93185
• NCBI Accession #: NP_001193594.1
• NCBI GenBank Nucleotide #: NM_001206665.2
• UniProt Accession #: Q969P0; Q8NG09; Q96DP4; Q9BTG9
• Molecular Weight: 55,808 Da
• NCBI Official Full Name: immunoglobulin superfamily member 8
• NCBI Official Synonym Full Names: immunoglobulin superfamily, member 8
• NCBI Official Symbol: IGSF8
• NCBI Official Synonym Symbols: EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
• NCBI Protein Information: immunoglobulin superfamily member 8
• UniProt Protein Name: Immunoglobulin superfamily member 8
• Protein Family: Immunoglobulin superfamily
• UniProt Gene Name: IGSF8
• UniProt Synonym Gene Names: CD81P3; EWI2; KCT4; IgSF8; EWI-2; KCT-4; PGRL
• UniProt Entry Name: IGSF8_HUMAN

NCBI Description

This gene encodes a member the EWI subfamily of the immunoglobulin protein superfamily. Members of this family contain a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a variable number of immunoglobulin domains. This protein interacts with the tetraspanins CD81 and CD9 and may regulate their role in certain cellular functions including cell migration and viral infection. The encoded protein may also function as a tumor suppressor by inhibiting the proliferation of certain cancers. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

IGSF8: May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin- dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: membrane; axon; integral to membrane; plasma membrane; synapse

Molecular Function: protein binding

Biological Process: nervous system development; cell proliferation; skeletal muscle development; single fertilization; cell motility