IFT88 sirna

IFT88 siRNA (Human)

Cat. Num. AAA8231797

• Gene Names: IFT88; DAF19; TG737; TTC10; hTg737; D13S1056E
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IFT88; IFT88 siRNA (Human); TG737; TTC10; Intraflagellar transport protein 88 homolog; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10; TPR repeat protein 10; IFT88 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IFT88 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 824

• Applicable Applications for IFT88 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IFT88 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IFT88 sirna

siRNA to inhibit IFT88 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28329439
• NCBI GeneID: 8100
• NCBI Accession #: NP_006522.2
• NCBI GenBank Nucleotide #: NM_006531.3
• UniProt Accession #: Q13099; Q5SZJ6; Q8N719; A2A491; B4DUS2
• Molecular Weight: 92,381 Da
• NCBI Official Full Name: intraflagellar transport protein 88 homolog isoform 2
• NCBI Official Synonym Full Names: intraflagellar transport 88
• NCBI Official Symbol: IFT88
• NCBI Official Synonym Symbols: DAF19; TG737; TTC10; hTg737; D13S1056E
• NCBI Protein Information: intraflagellar transport protein 88 homolog
• UniProt Protein Name: Intraflagellar transport protein 88 homolog
• Protein Family: Intraflagellar transport protein
• UniProt Gene Name: IFT88
• UniProt Synonym Gene Names: TG737; TTC10; TPR repeat protein 10
• UniProt Entry Name: IFT88_HUMAN

NCBI Description

This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TTC10: Involved in primary cilium biogenesis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 13q12.1

Cellular Component: centriole; apical part of cell; motile primary cilium; photoreceptor connecting cilium; acrosomal membrane; cilium

Molecular Function: kinesin binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; Notch signaling pathway; smoothened signaling pathway; epidermis development; regulation of fat cell differentiation; organelle organization and biogenesis; spinal cord dorsal/ventral patterning; palate development; forebrain morphogenesis; liver development; cardiac muscle cell differentiation; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; telencephalon development; protein localization; inner ear receptor stereocilium organization and biogenesis; spermatid nuclear elongation; eye development; pancreas development; sperm axoneme assembly; embryonic digit morphogenesis; kidney development; determination of left/right symmetry; negative regulation of epithelial cell proliferation; regulation of odontogenesis of dentine-containing teeth; regulation of smoothened signaling pathway