IFT122 sirna

IFT122 siRNA (Human)

Cat. Num. AAA8200145

• Gene Names: IFT122; CED; SPG; CED1; WDR10; WDR10p; WDR140
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IFT122; IFT122 siRNA (Human); SPG; WDR10; WDR140; Intraflagellar transport protein 122 homolog; WD repeat-containing protein 10; WD repeat-containing protein 140; IFT122 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IFT122 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1234

• Applicable Applications for IFT122 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IFT122 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IFT122 sirna

siRNA to inhibit IFT122 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 526253054
• NCBI GeneID: 55764
• NCBI Accession #: NP_001267470.1
• NCBI GenBank Nucleotide #: NM_001280541.1
• UniProt Accession #: Q9HBG6; Q53G36; Q8TC06; Q9BTB9; B3KW53; B4DEY9; B4DPW7; E7EQF4; E9PDG2; E9PDX2; G3XAB1; H7C3C0
• Molecular Weight: 113,230 Da
• NCBI Official Full Name: intraflagellar transport protein 122 homolog isoform 5
• NCBI Official Synonym Full Names: intraflagellar transport 122
• NCBI Official Symbol: IFT122
• NCBI Official Synonym Symbols: CED; SPG; CED1; WDR10; WDR10p; WDR140
• NCBI Protein Information: intraflagellar transport protein 122 homolog
• UniProt Protein Name: Intraflagellar transport protein 122 homolog
• Protein Family: Intraflagellar transport protein
• UniProt Gene Name: IFT122
• UniProt Synonym Gene Names: SPG; WDR10; WDR140
• UniProt Entry Name: IF122_HUMAN

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Mutations in this gene cause cranioectodermal dysplasia-1. A related pseudogene is located on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

IFT122: Required for cilia formation and Shh signaling during neuronal patterning. Defects in IFT122 are a cause of cranioectodermal dysplasia type 1 (CED1). CED1 is a disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include dolichocephaly (with or without sagittal suture synostosis), scaphocephaly, short stature, limb shortening, short ribs, narrow chest, brachydactyly, renal failure and hepatic fibrosis, small and abnormally shaped teeth, sparse hair, skin laxity and abnormal nails. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: membrane; cytoplasm; photoreceptor connecting cilium; cilium

Molecular Function: protein binding

Biological Process: limb development; embryonic forelimb morphogenesis; organelle organization and biogenesis; signal transduction downstream of smoothened; embryonic body morphogenesis; camera-type eye morphogenesis; negative regulation of smoothened signaling pathway; neural tube closure; cilium biogenesis; embryonic heart tube development; embryonic digit morphogenesis; negative regulation of epithelial cell proliferation; negative regulation of smoothened signaling pathway in ventral spinal cord patterning

Disease: Cranioectodermal Dysplasia 1