IFI16 sirna

IFI16 siRNA (Human)

Cat. Num. AAA8214484

• Gene Names: IFI16; PYHIN2; IFNGIP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IFI16; IFI16 siRNA (Human); IFNGIP1; Gamma-interferon-inducible protein 16; Ifi-16; Interferon-inducible myeloid differentiation transcriptional activator; IFI16 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IFI16 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 729

• Applicable Applications for IFI16 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IFI16 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IFI16 sirna

siRNA to inhibit IFI16 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 330864753
• NCBI GeneID: 3428
• NCBI Accession #: NP_001193496.1
• NCBI GenBank Nucleotide #: NM_001206567.1
• UniProt Accession #: Q16666; Q59GX0; Q5T3W7; Q5T3W8; Q5T3X0; Q5T3X1; Q5T3X2; Q8N9E5; Q8NEQ7; Q96AJ5; B4DJT8; H3BLV7
• Molecular Weight: 82,588 Da
• NCBI Official Full Name: gamma-interferon-inducible protein 16 isoform 1
• NCBI Official Synonym Full Names: interferon, gamma-inducible protein 16
• NCBI Official Symbol: IFI16
• NCBI Official Synonym Symbols: PYHIN2; IFNGIP1
• NCBI Protein Information: gamma-interferon-inducible protein 16
• UniProt Protein Name: Gamma-interferon-inducible protein 16
• Protein Family: Gamma-interferon-inducible protein
• UniProt Gene Name: IFI16
• UniProt Synonym Gene Names: IFNGIP1; Ifi-16
• UniProt Entry Name: IF16_HUMAN

NCBI Description

This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

IFI16: a nuclear protein that may function as a transcriptional repressor. Strongly induced by gamma interferon and, to a lesser extent, by alpha interferon. Binds double-stranded DNA and cell cycle regulatory factors including p53 and Rb. Loss of IFI16 activates p53 checkpoint through NBS1-DNAPK pathway. Inhibits cell growth in the Ras/Raf signaling pathway. May be involved in the senescence of prostate epithelial cells. Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prostate, ovary and breast. Four alternatively spliced isoforms have been described. Isoforms-1, -2 and -3 can homo- and hetero-dimerize.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nuclear speck; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; double-stranded DNA binding; transcription factor binding

Biological Process: positive regulation of cytokine production; monocyte differentiation; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of DNA binding; positive regulation of interleukin-1 beta production; cell proliferation; cellular response to glucose starvation; negative regulation of viral genome replication; negative regulation of innate immune response; positive regulation of interferon type I production; innate immune response; autophagy; hemopoiesis; myeloid cell differentiation; regulation of autophagy; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of transcription, DNA-dependent; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; activation of innate immune response