HSD3B2 sirna

HSD3B2 siRNA (Human)

Cat. Num. AAA8216067

• Gene Names: HSD3B2; HSDB; HSD3B; SDR11E2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HSD3B2; HSD3B2 siRNA (Human); HSDB3B; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3-beta-HSD II; 3-beta-HSD adrenal and gonadal type; HSD3B2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HSD3B2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 222

• Applicable Applications for HSD3B2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HSD3B2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HSD3B2 sirna

siRNA to inhibit HSD3B2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4504509
• NCBI GeneID: 3284
• NCBI Accession #: NP_000189.1
• NCBI GenBank Nucleotide #: NM_000198.3
• UniProt Accession #: P26439; Q16010; Q53GD4; Q6AI10; Q6LDB9; Q99890; Q9UD08; A2RRA5
• Molecular Weight: 24,987 Da
• NCBI Official Full Name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
• NCBI Official Synonym Full Names: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
• NCBI Official Symbol: HSD3B2
• NCBI Official Synonym Symbols: HSDB; HSD3B; SDR11E2
• NCBI Protein Information: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
• UniProt Protein Name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
• Protein Family: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
• UniProt Gene Name: HSD3B2
• UniProt Synonym Gene Names: HSDB3B; 3-beta-HSD II
• UniProt Entry Name: 3BHS2_HUMAN

NCBI Description

The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

HSD3B2: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Lipid Metabolism - androgen and estrogen; EC 5.3.3.1; EC 1.1.1.145; Isomerase; Endoplasmic reticulum; Membrane protein, integral; Mitochondrial; Oxidoreductase; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; mitochondrial inner membrane; mitochondrial membrane; integral to membrane; mitochondrial intermembrane space; smooth endoplasmic reticulum membrane

Molecular Function: steroid delta-isomerase activity; 3-beta-hydroxy-delta5-steroid dehydrogenase activity

Biological Process: steroid metabolic process; mineralocorticoid biosynthetic process; androgen biosynthetic process; glucocorticoid biosynthetic process; steroid biosynthetic process

Disease: 3-beta-hydroxysteroid Dehydrogenase, Type Ii, Deficiency Of