HOXD10 sirna

HOXD10 siRNA (Human)

Cat. Num. AAA8201099

• Gene Names: HOXD10; HOX4; HOX4D; HOX4E; Hox-4.4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HOXD10; HOXD10 siRNA (Human); HOX4D; HOX4E; Homeobox protein Hox-D10; Homeobox protein Hox-4D; Homeobox protein Hox-4E; HOXD10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HOXD10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 340

• Applicable Applications for HOXD10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HOXD10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HOXD10 sirna

siRNA to inhibit HOXD10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23510366
• NCBI GeneID: 3236
• NCBI Accession #: NP_002139.2
• NCBI GenBank Nucleotide #: NM_002148.3
• UniProt Accession #: P28358; Q6NT10
• Molecular Weight: 38,411 Da
• NCBI Official Full Name: homeobox protein Hox-D10
• NCBI Official Synonym Full Names: homeobox D10
• NCBI Official Symbol: HOXD10
• NCBI Official Synonym Symbols: HOX4; HOX4D; HOX4E; Hox-4.4
• NCBI Protein Information: homeobox protein Hox-D10
• UniProt Protein Name: Homeobox protein Hox-D10
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXD10
• UniProt Synonym Gene Names: HOX4D; HOX4E
• UniProt Entry Name: HXD10_HUMAN

NCBI Description

This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. Mutations in this gene have been associated with Wilm's tumor and congenital vertical talus (also known as "rocker-bottom foot" deformity or congenital convex pes valgus) and/or a foot deformity resembling that seen in Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]

Uniprot Description

HOXD10: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Defects in HOXD10 are a cause of congenital vertical talus (CVT); also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity. Belongs to the Abd-B homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: hindlimb morphogenesis; skeletal muscle development; transcription, DNA-dependent; adult locomotory behavior; neuromuscular process; spinal cord motor neuron cell fate specification; embryonic skeletal morphogenesis; peripheral nervous system neuron development; anterior/posterior pattern formation; forelimb morphogenesis; single fertilization; positive regulation of transcription from RNA polymerase II promoter; proximal/distal pattern formation; embryonic limb morphogenesis

Disease: Vertical Talus, Congenital