HOGA1 sirna

HOGA1 siRNA (Human)

Cat. Num. AAA8214625

• Gene Names: HOGA1; HP3; NPL2; DHDPS2; DHDPSL; C10orf65
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HOGA1; HOGA1 siRNA (Human); C10orf65; DHDPSL; 4-hydroxy-2-oxoglutarate aldolase mitochondrial; Dihydrodipicolinate synthase-like; DHDPS-like protein; Probable 2-keto-4-hydroxyglutarate aldolase; Probable KHG-aldolase; Protein 569272; HOGA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HOGA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 164

• Applicable Applications for HOGA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HOGA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HOGA1 sirna

siRNA to inhibit HOGA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 197927274
• NCBI GeneID: 112817
• NCBI Accession #: NP_001128142.1
• NCBI GenBank Nucleotide #: NM_001134670.1
• UniProt Accession #: Q86XE5; Q5T680; Q5T684; Q711P0; Q8N9F2; Q96EV5; A8K075
• Molecular Weight: 17,954 Da
• NCBI Official Full Name: 4-hydroxy-2-oxoglutarate aldolase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: 4-hydroxy-2-oxoglutarate aldolase 1
• NCBI Official Symbol: HOGA1
• NCBI Official Synonym Symbols: HP3; NPL2; DHDPS2; DHDPSL; C10orf65
• NCBI Protein Information: 4-hydroxy-2-oxoglutarate aldolase, mitochondrial
• UniProt Protein Name: 4-hydroxy-2-oxoglutarate aldolase, mitochondrial
• Protein Family: 4-hydroxy-2-oxoglutarate aldolase
• UniProt Gene Name: HOGA1
• UniProt Synonym Gene Names: C10orf65; DHDPSL; DHDPS-like protein; Probable KHG-aldolase
• UniProt Entry Name: HOGA1_HUMAN

NCBI Description

The authors of PMID:20797690 cloned this gene while searching for genes in a region of chromosome 10 linked to primary hyperoxalurea type III. They noted that even though the encoded protein has been described as a mitochondrial dihydrodipicolinate synthase-like enzyme, it shares little homology with E. coli dihydrodipicolinate synthase (Dhdps), particularly in the putative substrate-binding region. Moreover, neither lysine biosynthesis nor sialic acid metabolism, for which Dhdps is responsible, occurs in vertebrate mitochondria. They propose that this gene encodes mitochondrial 4-hydroxyl-2-oxoglutarate aldolase (EC 4.1.3.16), which catalyzes the final step in the metabolic pathway of hydroxyproline, releasing glyoxylate and pyruvate. This gene is predominantly expressed in the liver and kidney, and mutations in this gene are found in patients with primary hyperoxalurea type III. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

DHDPSL: Catalyzes the final step in the metabolic pathway of hydroxyproline (Probable). Defects in HOGA1 are the cause of hyperoxaluria primary type 3 (HP3); also known as primary hyperoxaluria type III (PH3). HP3 is a disorder where the main clinical manifestation is calcium oxalate nephrolithiasis though chronic as well as terminal renal insufficiency has been described. It is characterized by an elevated urinary excretion of oxalate and L- glycerate. Belongs to the DHDPS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 4.1.3.16; Lyase

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: mitochondrion

Molecular Function: protein homodimerization activity; 4-hydroxy-2-oxoglutarate aldolase activity

Biological Process: pyruvate biosynthetic process; glyoxylate catabolic process; 4-hydroxyproline catabolic process; glyoxylate metabolic process; oxalate metabolic process

Disease: Hyperoxaluria, Primary, Type Iii