HNRNPH3 sirna

HNRNPH3 siRNA (Human)

Cat. Num. AAA8213618

• Gene Names: HNRNPH3; 2H9; HNRPH3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HNRNPH3; HNRNPH3 siRNA (Human); HNRPH3; Heterogeneous nuclear ribonucleoprotein H3; hnRNP H3; Heterogeneous nuclear ribonucleoprotein 2H9; hnRNP 2H9; HNRNPH3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HNRNPH3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 346

• Applicable Applications for HNRNPH3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HNRNPH3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HNRNPH3 sirna

siRNA to inhibit HNRNPH3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14141157
• NCBI GeneID: 3189
• NCBI Accession #: NP_036339.1
• NCBI GenBank Nucleotide #: NM_012207.2
• UniProt Accession #: P31942; Q9BSX1; Q9NP53; Q9NP96; Q9NPA7; Q9NPI4; Q9UFU4; Q9Y4J5; A8K682; B3KRE1
• Molecular Weight: 15,413 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein H3 isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein H3 (2H9)
• NCBI Official Symbol: HNRNPH3
• NCBI Official Synonym Symbols: 2H9; HNRPH3
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein H3
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein H3
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPH3
• UniProt Synonym Gene Names: HNRPH3; hnRNP H3; hnRNP 2H9
• UniProt Entry Name: HNRH3_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is localized in nuclear bodies of the nucleus. This protein is involved in the splicing process and it also participates in early heat shock-induced splicing arrest by transiently leaving the hnRNP complexes. Several alternatively spliced transcript variants have been noted for this gene, however, not all are fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP 2H9: Involved in the splicing process and participates in early heat shock-induced splicing arrest. Due to their great structural variations the different isoforms may possess different functions in the splicing reaction. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: nucleoplasm; spliceosome; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA processing; epithelial cell differentiation; RNA splicing