HMCN1 sirna

HMCN1 siRNA (Human)

Cat. Num. AAA8228967

• Gene Names: HMCN1; ARMD1; FBLN6; FIBL6; FIBL-6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HMCN1; HMCN1 siRNA (Human); FIBL6; Hemicentin-1; Fibulin-6; FIBL-6; HMCN1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HMCN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 5635

• Applicable Applications for HMCN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HMCN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HMCN1 sirna

siRNA to inhibit HMCN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 118572606
• NCBI GeneID: 83872
• NCBI Accession #: NP_114141.2
• NCBI GenBank Nucleotide #: NM_031935.2
• UniProt Accession #: Q96RW7; Q5TYR7; Q96DN3; Q96DN8; Q96SC3; A6NGE3
• Molecular Weight: 59,747 Da
• NCBI Official Full Name: hemicentin-1
• NCBI Official Synonym Full Names: hemicentin 1
• NCBI Official Symbol: HMCN1
• NCBI Official Synonym Symbols: ARMD1; FBLN6; FIBL6; FIBL-6
• NCBI Protein Information: hemicentin-1
• UniProt Protein Name: Hemicentin-1
• Protein Family: Hemicentin
• UniProt Gene Name: HMCN1
• UniProt Synonym Gene Names: FIBL6; FIBL-6
• UniProt Entry Name: HMCN1_HUMAN

NCBI Description

This gene encodes a large extracellular member of the immunoglobulin superfamily. A similar protein in C. elegans forms long, fine tracks at specific extracellular sites that are involved in many processes such as stabilization of the germline syncytium, anchorage of mechanosensory neurons to the epidermis, and organization of hemidesmosomes in the epidermis. Mutations in this gene may be associated with age-related macular degeneration. [provided by RefSeq, Jul 2008]

Uniprot Description

HMCN1: Defects in HMCN1 are a cause of age-related macular degeneration type 1 (ARMD1). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25.3-q31.1

Cellular Component: extracellular matrix; basement membrane; cell cortex; cell junction

Molecular Function: calcium ion binding

Biological Process: visual perception; response to stimulus

Disease: Macular Degeneration, Age-related, 1