HERC5 sirna

HERC5 siRNA (Human)

Cat. Num. AAA8203798

• Gene Names: HERC5; CEB1; CEBP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HERC5; HERC5 siRNA (Human); CEB1; CEBP1; E3 ISG15--protein ligase HERC5; Cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5; HERC5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HERC5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1024

• Applicable Applications for HERC5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HERC5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HERC5 sirna

siRNA to inhibit HERC5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110825982
• NCBI GeneID: 51191
• NCBI Accession #: NP_057407.2
• NCBI GenBank Nucleotide #: NM_016323.3
• UniProt Accession #: Q9UII4; Q69G20; B2RTQ1
• Molecular Weight: 116,852 Da
• NCBI Official Full Name: E3 ISG15--protein ligase HERC5
• NCBI Official Synonym Full Names: HECT and RLD domain containing E3 ubiquitin protein ligase 5
• NCBI Official Symbol: HERC5
• NCBI Official Synonym Symbols: CEB1; CEBP1
• NCBI Protein Information: E3 ISG15--protein ligase HERC5
• UniProt Protein Name: E3 ISG15--protein ligase HERC5
• Protein Family: E3 ISG15--protein ligase
• UniProt Gene Name: HERC5
• UniProt Synonym Gene Names: CEB1; CEBP1
• UniProt Entry Name: HERC5_HUMAN

NCBI Description

This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

HERC5: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: ISG15 conjugating enzyme activity; protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ISG15-protein conjugation; cytokine and chemokine mediated signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of defense response to virus; innate immune response; regulation of cyclin-dependent protein kinase activity; defense response to virus; negative regulation of interferon type I production