Rabbit anti-Human, Mouse PICK1 Polyclonal Antibody | anti-PICK1 antibody

PICK1, CT (Protein Interacting with PRKCA 1, Protein Interacting with C Kinase 1, PICK-1, PICK, MGC15204, Protein Kinase C alpha Binding Protein, PRKCA Binding Protein, Prkcabp) (Azide free) (HRP)

Cat. Num. AAA6493392

• Gene Names: PICK1; PICK; PRKCABP
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PICK1; Polyclonal Antibody; CT (Protein Interacting with PRKCA 1; Protein Interacting with C Kinase 1; PICK-1; PICK; MGC15204; Protein Kinase C alpha Binding Protein; PRKCA Binding Protein; Prkcabp) (Azide free) (HRP); Protein that Interacts with C Kinase 1; OTTHUMP00000028509; anti-PICK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKCABP. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PICK1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PRKCABP (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hPRKCABP-C300 in HL-60, Jurkat, K562 cell line lysates (35ug/lane). PRKCABP (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-PICK1 antibody

Antibodies; Protein Kinases

References

1. Lei Xue, et al. Am J Physiol Cell Physiol, Jun 2010; 298: C1572-C1582. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 22095990
• NCBI GeneID: 9463
• UniProt Accession #: Q9NRD5; O95906; B3KS52
• Molecular Weight: 415
• NCBI Official Full Name: PRKCA-binding protein
• NCBI Official Synonym Full Names: protein interacting with PRKCA 1
• NCBI Official Symbol: PICK1
• NCBI Official Synonym Symbols: PICK; PRKCABP
• NCBI Protein Information: PRKCA-binding protein; protein interacting with C kinase 1; protein kinase C-alpha-binding protein
• UniProt Protein Name: PRKCA-binding protein
• Protein Family: PRKCA-binding protein
• UniProt Gene Name: PICK1
• UniProt Synonym Gene Names: PRKCABP
• UniProt Entry Name: PICK1_HUMAN

NCBI Description

The protein encoded by this gene contains a PDZ domain, through which it interacts with protein kinase C, alpha (PRKCA). This protein may function as an adaptor that binds to and organizes the subcellular localization of a variety of membrane proteins. It has been shown to interact with multiple glutamate receptor subtypes, monoamine plasma membrane transporters, as well as non-voltage gated sodium channels, and may target PRKCA to these membrane proteins and thus regulate their distribution and function. This protein has also been found to act as an anchoring protein that specifically targets PRKCA to mitochondria in a ligand-specific manner. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PICK1: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ASIC1/ASIC3 channel.

Protein type: Cell development/differentiation; Ligand, receptor tyrosine kinase; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: presynaptic membrane; Golgi apparatus; postsynaptic membrane; neuron projection; cytoskeleton; mitochondrion; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; cell junction

Molecular Function: actin filament binding; protein C-terminus binding; identical protein binding; protein domain specific binding; protein binding; enzyme binding; G-protein-coupled receptor binding; protein kinase C binding; metal ion binding; ATPase activity; receptor binding

Biological Process: glial cell development; cellular response to glucose starvation; synaptic transmission; DNA methylation during gametogenesis; monoamine transport; positive regulation of receptor internalization; protein kinase C activation; retrograde vesicle-mediated transport, Golgi to ER; DNA methylation during embryonic development; neuronal ion channel clustering; protein amino acid phosphorylation; receptor clustering; protein targeting