HERC2 sirna

HERC2 siRNA (Human)

Cat. Num. AAA8226635

• Gene Names: HERC2; jdf2; p528; MRT38; SHEP1; D15F37S1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HERC2; HERC2 siRNA (Human); E3 ubiquitin-protein ligase HERC2; HECT domain and RCC1-like domain-containing protein 2; HERC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HERC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 4,834

• Applicable Applications for HERC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HERC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HERC2 sirna

siRNA to inhibit HERC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 126032348
• NCBI GeneID: 8924
• NCBI Accession #: NP_004658.3
• NCBI GenBank Nucleotide #: NM_004667.5
• UniProt Accession #: O95714; Q86SV7; Q86SV8; Q86SV9; Q86YY3; Q86YY4; Q86YY5; Q86YY6; Q86YY7; Q86YY8; Q86YY9; Q86YZ0
• Molecular Weight: 527,228 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase HERC2
• NCBI Official Synonym Full Names: HECT and RLD domain containing E3 ubiquitin protein ligase 2
• NCBI Official Symbol: HERC2
• NCBI Official Synonym Symbols: jdf2; p528; MRT38; SHEP1; D15F37S1
• NCBI Protein Information: E3 ubiquitin-protein ligase HERC2
• UniProt Protein Name: E3 ubiquitin-protein ligase HERC2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: HERC2
• UniProt Entry Name: HERC2_HUMAN

NCBI Description

This gene belongs to the HERC gene family that encodes a group of unusually large proteins, which contain multiple structural domains. All members have at least 1 copy of an N-terminal region showing homology to the cell cycle regulator RCC1 and a C-terminal HECT (homologous to E6-AP C terminus) domain found in a number of E3 ubiquitin protein ligases. Genetic variations in this gene are associated with skin/hair/eye pigmentation variability. Multiple pseudogenes of this gene are located on chromosomes 15 and 16. [provided by RefSeq, Mar 2012]

Uniprot Description

HERC2: E3 ubiquitin-protein ligase that regulates ubiquitin- dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. Interacts (when phosphorylated at Thr-4827) with RNF8 (via FHA domain); this interaction increases after ionising radiation (IR) treatment. Interacts with XPA.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; Mitochondrial

Chromosomal Location of Human Ortholog: 15q13

Cellular Component: centriole; membrane; cytoplasm; mitochondrial inner membrane; nucleus

Molecular Function: protein binding; SUMO binding; zinc ion binding; guanyl-nucleotide exchange factor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; heme binding; ligase activity

Biological Process: intracellular protein transport; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; spermatogenesis; DNA repair; response to DNA damage stimulus; positive regulation of GTPase activity

Disease: Prader-willi Syndrome; Mental Retardation, Autosomal Recessive 38; Skin/hair/eye Pigmentation, Variation In, 1