GPT2 sirna

GPT2 siRNA (Human)

Cat. Num. AAA8230785

• Gene Names: GPT2; ALT2; GPT 2; MRT49
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GPT2; GPT2 siRNA (Human); AAT2; ALT2; Alanine aminotransferase 2; Glutamate pyruvate transaminase 2; GPT 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2; GPT2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GPT2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 423

• Applicable Applications for GPT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GPT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GPT2 sirna

siRNA to inhibit GPT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 215599424
• NCBI GeneID: 84706
• NCBI Accession #: NP_001135938.1
• NCBI GenBank Nucleotide #: NM_001142466.2
• UniProt Accession #: Q8TD30; Q8N9E2
• Molecular Weight: 46,983 Da
• NCBI Official Full Name: alanine aminotransferase 2 isoform 2
• NCBI Official Synonym Full Names: glutamic pyruvate transaminase (alanine aminotransferase) 2
• NCBI Official Symbol: GPT2
• NCBI Official Synonym Symbols: ALT2; GPT 2; MRT49
• NCBI Protein Information: alanine aminotransferase 2
• UniProt Protein Name: Alanine aminotransferase 2
• Protein Family: Glycerol-3-phosphate O-acyltransferase
• UniProt Gene Name: GPT2
• UniProt Synonym Gene Names: AAT2; ALT2; ALT2; GPT 2
• UniProt Entry Name: ALAT2_HUMAN

NCBI Description

This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

GPT2: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.6.1.2; Mitochondrial; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: mitochondrial matrix

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine metabolic process; L-alanine catabolic process; 2-oxoglutarate metabolic process

Disease: Mental Retardation, Autosomal Recessive 49