GOSR2 sirna

GOSR2 siRNA (Human)

Cat. Num. AAA829589

• Gene Names: GOSR2; Bos1; EPM6; GS27
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GOSR2; GOSR2 siRNA (Human); GS27; Golgi SNAP receptor complex member 2; 27 kDa Golgi SNARE protein; Membrin; GOSR2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GOSR2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 195

• Applicable Applications for GOSR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GOSR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GOSR2 sirna

siRNA to inhibit GOSR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 60499013
• NCBI GeneID: 9570
• NCBI Accession #: NP_001012529.1
• NCBI GenBank Nucleotide #: NM_001012511.1
• UniProt Accession #: O14653; Q8N4B8; Q96DA5; Q9BZZ4; D3DXJ5; D3DXJ6
• Molecular Weight: 22,725 Da
• NCBI Official Full Name: Golgi SNAP receptor complex member 2 isoform C
• NCBI Official Synonym Full Names: golgi SNAP receptor complex member 2
• NCBI Official Symbol: GOSR2
• NCBI Official Synonym Symbols: Bos1; EPM6; GS27
• NCBI Protein Information: Golgi SNAP receptor complex member 2
• UniProt Protein Name: Golgi SNAP receptor complex member 2
• Protein Family: Golgi SNAP receptor complex
• UniProt Gene Name: GOSR2
• UniProt Synonym Gene Names: GS27
• UniProt Entry Name: GOSR2_HUMAN

NCBI Description

This gene encodes a trafficking membrane protein which transports proteins among the medial- and trans-Golgi compartments. Due to its chromosomal location and trafficking function, this gene may be involved in familial essential hypertension. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GOSR2: Involved in transport of proteins from the cis/medial- Golgi to the trans-Golgi network. Defects in GOSR2 are the cause of progressive myoclonic epilepsy type 6 (EPM6). A neurologic disorder characterized by onset of ataxia in the first years of life, followed by action myoclonus and seizures later in childhood, and loss of independent ambulation in the second decade. Cognition is not usually affected, although mild memory difficulties may occur in the third decade. Belongs to the GOSR2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: SNARE complex; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; Golgi stack; membrane; late endosome membrane; integral to membrane

Molecular Function: SNAP receptor activity; SNARE binding; transporter activity

Biological Process: intra-Golgi vesicle-mediated transport; ER to Golgi vesicle-mediated transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response; Golgi to vacuole transport; vesicle fusion with Golgi apparatus; protein targeting to vacuole; retrograde transport, endosome to Golgi

Disease: Epilepsy, Progressive Myoclonic 6