GMEB2 sirna

GMEB2 siRNA (Human)

Cat. Num. AAA8202852

• Gene Names: GMEB2; PIF79; P79PIF
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GMEB2; GMEB2 siRNA (Human); KIAA1269; Glucocorticoid modulatory element-binding protein 2; GMEB-2; DNA-binding protein p79PIF; Parvovirus initiation factor p79; PIF p79; GMEB2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GMEB2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 530

• Applicable Applications for GMEB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GMEB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GMEB2 sirna

siRNA to inhibit GMEB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6912568
• NCBI GeneID: 26205
• NCBI Accession #: NP_036516.1
• NCBI GenBank Nucleotide #: NM_012384.3
• UniProt Accession #: Q9UKD1; Q5TDS0; Q9H431; Q9H4X7; Q9H4X8; Q9UF78; Q9ULF1; E1P5J3
• Molecular Weight: 56,421 Da
• NCBI Official Full Name: glucocorticoid modulatory element-binding protein 2
• NCBI Official Synonym Full Names: glucocorticoid modulatory element binding protein 2
• NCBI Official Symbol: GMEB2
• NCBI Official Synonym Symbols: PIF79; P79PIF
• NCBI Protein Information: glucocorticoid modulatory element-binding protein 2
• UniProt Protein Name: Glucocorticoid modulatory element-binding protein 2
• Protein Family: Glucocorticoid modulatory element-binding protein
• UniProt Gene Name: GMEB2
• UniProt Synonym Gene Names: KIAA1269; GMEB-2; PIF p79
• UniProt Entry Name: GMEB2_HUMAN

NCBI Description

This gene is a member of KDWK gene family. The product of this gene associates with GMEB1 protein, and the complex is essential for parvovirus DNA replication. Study of rat homolog implicates the role of this gene in modulation of transactivation by the glucocorticoid receptor bound to glucocorticoid response elements. This gene appears to use multiple polyadenylation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

GMEB2: Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent