GHRHR sirna

GHRHR siRNA (Human)

Cat. Num. AAA8213630

• Gene Names: GHRHR; GRFR; GHRFR; IGHD1B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GHRHR; GHRHR siRNA (Human); Growth hormone-releasing hormone receptor; GHRH receptor; Growth hormone-releasing factor receptor; GRF receptor; GRFR; GHRHR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GHRHR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 423

• Applicable Applications for GHRHR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GHRHR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GHRHR sirna

siRNA to inhibit GHRHR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58530851
• NCBI GeneID: 2692
• NCBI Accession #: NP_000814.2
• NCBI GenBank Nucleotide #: NM_000823.3
• UniProt Accession #: Q02643; Q99863
• Molecular Weight: 47,402 Da
• NCBI Official Full Name: growth hormone-releasing hormone receptor
• NCBI Official Synonym Full Names: growth hormone releasing hormone receptor
• NCBI Official Symbol: GHRHR
• NCBI Official Synonym Symbols: GRFR; GHRFR; IGHD1B
• NCBI Protein Information: growth hormone-releasing hormone receptor
• UniProt Protein Name: Growth hormone-releasing hormone receptor
• Protein Family: Growth hormone-releasing hormone receptor
• UniProt Gene Name: GHRHR
• UniProt Synonym Gene Names: GHRH receptor; GRF receptor; GRFR
• UniProt Entry Name: GHRHR_HUMAN

NCBI Description

This gene encodes a receptor for growth hormone-releasing hormone. Binding of this hormone to the receptor leads to synthesis and release of growth hormone. Mutations in this gene have been associated with isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, a disorder characterized by short stature. [provided by RefSeq, Jun 2010]

Uniprot Description

GHRHR: Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Belongs to the G-protein coupled receptor 2 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear outer membrane; nuclear matrix; cell surface; cytoplasm; integral to membrane; plasma membrane; nuclear inner membrane; secretory granule

Molecular Function: G-protein coupled receptor activity; protein binding; peptide hormone binding; growth factor binding; growth hormone-releasing hormone receptor activity

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; lactation; regulation of protein metabolic process; cell maturation; adenylate cyclase activation; response to glucocorticoid stimulus; determination of adult life span; positive regulation of multicellular organism growth; reproductive process in a multicellular organism; G-protein signaling, adenylate cyclase activating pathway; response to insulin stimulus; positive regulation of growth hormone secretion; water homeostasis; growth hormone secretion; cAMP-mediated signaling; cellular response to insulin stimulus; response to estrogen stimulus; positive regulation of cell proliferation; somatotropin secreting cell development; positive regulation of cAMP biosynthetic process; hormone metabolic process; regulation of steroid hormone receptor signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Ib