GCNT2 sirna

GCNT2 siRNA (Human)

Cat. Num. AAA8220724

• Gene Names: GCNT2; II; CCAT; IGNT; ULG3; GCNT5; GCNT2C; NACGT1; NAGCT1; CTRCT13; bA421M1.1; bA360O19.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GCNT2; GCNT2 siRNA (Human); GCNT5; II; NACGT1; N-acetyllactosaminide beta-1.6-N-acetylglucosaminyl-transferase isoform B; N-acetylglucosaminyltransferase; I-branching enzyme; IGNT; GCNT2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GCNT2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 400

• Applicable Applications for GCNT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GCNT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GCNT2 sirna

siRNA to inhibit GCNT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503963
• NCBI GeneID: 2651
• NCBI Accession #: NP_001482.1
• NCBI GenBank Nucleotide #: NM_001491.2
• UniProt Accession #: Q06430; Q5W0E9; Q8N0V5; Q8NFS9
• Molecular Weight: 45,855 Da
• NCBI Official Full Name: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase isoform B
• NCBI Official Synonym Full Names: glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
• NCBI Official Symbol: GCNT2
• NCBI Official Synonym Symbols: II; CCAT; IGNT; ULG3; GCNT5; GCNT2C; NACGT1; NAGCT1; CTRCT13; bA421M1.1; bA360O19.2
• NCBI Protein Information: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
• UniProt Protein Name: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase, isoform B
• Protein Family: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
• UniProt Gene Name: GCNT2
• UniProt Synonym Gene Names: GCNT5; II; NACGT1; N-acetylglucosaminyltransferase
• UniProt Entry Name: GNT2B_HUMAN

NCBI Description

This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

GCNT2B: Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells. The expression of the blood group I antigen in erythrocytes is determined by isoform C. Belongs to the glycosyltransferase 14 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.150; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p24.2

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity

Biological Process: glycosaminoglycan biosynthetic process; positive regulation of protein kinase B signaling cascade; positive regulation of heterotypic cell-cell adhesion; transforming growth factor beta receptor signaling pathway; multicellular organismal development; positive regulation of cell proliferation; protein amino acid glycosylation; positive regulation of cell migration

Disease: Blood Group, I System; Cataract 13 With Adult I Phenotype