G3BP1 sirna

G3BP1 siRNA (Human)

Cat. Num. AAA8232597

• Gene Names: G3BP1; G3BP; HDH-VIII
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: G3BP1; G3BP1 siRNA (Human); G3BP; Ras GTPase-activating protein-binding protein 1; G3BP-1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1; G3BP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: G3BP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 466

• Applicable Applications for G3BP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human G3BP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for G3BP1 sirna

siRNA to inhibit G3BP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5031703
• NCBI GeneID: 10146
• NCBI Accession #: NP_005745.1
• NCBI GenBank Nucleotide #: NM_005754.2
• UniProt Accession #: Q13283; Q5HYE9
• Molecular Weight: 13,874 Da
• NCBI Official Full Name: ras GTPase-activating protein-binding protein 1
• NCBI Official Synonym Full Names: GTPase activating protein (SH3 domain) binding protein 1
• NCBI Official Symbol: G3BP1
• NCBI Official Synonym Symbols: G3BP; HDH-VIII
• NCBI Protein Information: ras GTPase-activating protein-binding protein 1
• UniProt Protein Name: Ras GTPase-activating protein-binding protein 1
• Protein Family: Ras GTPase-activating protein-binding protein
• UniProt Gene Name: G3BP1
• UniProt Synonym Gene Names: G3BP; G3BP-1; hDH VIII
• UniProt Entry Name: G3BP1_HUMAN

NCBI Description

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

G3BP-1: an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Binds to the SH3 domain of Ras GTPase-activating protein (RasGAP) in proliferating cells but not in quiescent cells. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to SGs upon either arsenite or high temperature treatment. RasGAP-dependent phosphorylation of S149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. S149 phosphorylation induces partial nuclear localization. A component of a TAU mRNP complex, Interacts with USP10, and may regulate it.

Protein type: Helicase; RNA-binding; Adaptor/scaffold; EC 3.6.4.13; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: focal adhesion; stress granule; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: mRNA binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; endonuclease activity; ATP binding; ATP-dependent RNA helicase activity

Biological Process: metabolic process; transport; Ras protein signal transduction; DNA duplex unwinding