FNBP1L sirna

FNBP1L siRNA (Human)

Cat. Num. AAA8212138

• Gene Names: FNBP1L; TOCA1; C1orf39
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FNBP1L; FNBP1L siRNA (Human); C1orf39; TOCA1; Formin-binding protein 1-like; Transducer of Cdc42-dependent actin assembly protein 1; Toca-1; FNBP1L sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FNBP1L siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 551

• Applicable Applications for FNBP1L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FNBP1L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FNBP1L sirna

siRNA to inhibit FNBP1L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 68348709
• NCBI GeneID: 54874
• NCBI Accession #: NP_001020119.1
• NCBI GenBank Nucleotide #: NM_001024948.2
• UniProt Accession #: Q5T0N5; Q5T0N6; Q6B097; Q6P653; Q6R4Q4; Q9NXG1; J3QSS4
• Molecular Weight: 69,894 Da
• NCBI Official Full Name: formin-binding protein 1-like isoform 1
• NCBI Official Synonym Full Names: formin binding protein 1-like
• NCBI Official Symbol: FNBP1L
• NCBI Official Synonym Symbols: TOCA1; C1orf39
• NCBI Protein Information: formin-binding protein 1-like
• UniProt Protein Name: Formin-binding protein 1-like
• Protein Family: Formin-binding protein
• UniProt Gene Name: FNBP1L
• UniProt Synonym Gene Names: C1orf39; TOCA1; Toca-1
• UniProt Entry Name: FBP1L_HUMAN

NCBI Description

The protein encoded by this gene binds to both CDC42 and N-WASP. This protein promotes CDC42-induced actin polymerization by activating the N-WASP-WIP complex and, therefore, is involved in a pathway that links cell surface signals to the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FNBP1L: Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens. Belongs to the FNBP1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: cytoskeleton; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; cell cortex

Molecular Function: protein binding; lipid binding

Biological Process: positive regulation of filopodium formation; vesicle transport along actin filament; vesicle organization and biogenesis; membrane invagination; cilium biogenesis; autophagy; membrane budding