FKBP1B sirna

FKBP1B siRNA (Human)

Cat. Num. AAA8200653

• Gene Names: FKBP1B; OTK4; FKBP1L; PKBP1L; PPIase; FKBP12.6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FKBP1B; FKBP1B siRNA (Human); FKBP12.6; FKBP1L; FKBP9; OTK4; Peptidyl-prolyl cis-trans isomerase FKBP1B; PPIase FKBP1B; 12.6 kDa FK506-binding protein; 12.6 kDa FKBP; FKBP-12.6; FK506-binding protein 1B; FKBP-1B; Immunophilin FKBP12.6; Rotamase; h-FKBP-12; FKBP1B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FKBP1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 108

• Applicable Applications for FKBP1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FKBP1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FKBP1B sirna

siRNA to inhibit FKBP1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4758380
• NCBI GeneID: 2281
• NCBI Accession #: NP_004107.1
• NCBI GenBank Nucleotide #: NM_004116.3
• UniProt Accession #: P68106; Q13664; Q16645; Q53TM2; Q9BQ40
• Molecular Weight: 8,802 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP1B isoform a
• NCBI Official Synonym Full Names: FK506 binding protein 1B, 12.6 kDa
• NCBI Official Symbol: FKBP1B
• NCBI Official Synonym Symbols: OTK4; FKBP1L; PKBP1L; PPIase; FKBP12.6
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP1B
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP1B
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP1B
• UniProt Synonym Gene Names: FKBP12.6; FKBP1L; FKBP9; OTK4; PPIase FKBP1B; 12.6 kDa FKBP; FKBP-12.6; FKBP-1B
• UniProt Entry Name: FKB1B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. There are two alternatively spliced transcript variants of this gene encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FKBP1B: Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the FKBP-type PPIase family. FKBP1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.2.1.8; Isomerase

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: sarcoplasmic reticulum membrane; membrane; cytoplasm; cytosol; Z disc

Molecular Function: protein binding; FK506 binding; cyclic nucleotide binding; peptidyl-prolyl cis-trans isomerase activity; calcium channel inhibitor activity; ryanodine-sensitive calcium-release channel activity; receptor binding

Biological Process: positive regulation of sequestering of calcium ion; positive regulation of axon regeneration; protein maturation via protein folding; negative regulation of heart rate; protein peptidyl-prolyl isomerization; cytosolic calcium ion homeostasis; response to redox state; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; negative regulation of protein phosphatase type 2B activity; T cell proliferation; response to vitamin E; smooth muscle contraction; 'de novo' protein folding; response to hydrogen peroxide; insulin secretion; negative regulation of release of sequestered calcium ion into cytosol; action potential propagation; response to glucose stimulus; protein refolding; transmembrane transport