ERN1 sirna

ERN1 siRNA (Human)

Cat. Num. AAA8228765

• Gene Names: ERN1; IRE1; IRE1P; IRE1a; hIRE1p
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERN1; ERN1 siRNA (Human); IRE1; Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a; ERN1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ERN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 977

• Applicable Applications for ERN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ERN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERN1 sirna

siRNA to inhibit ERN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 153946421
• NCBI GeneID: 2081
• NCBI Accession #: NP_001424.3
• NCBI GenBank Nucleotide #: NM_001433.3
• UniProt Accession #: O75460; Q59EE2; A1L457; A8K8N8; A8MXS7
• Molecular Weight: 6,649 Da
• NCBI Official Full Name: serine/threonine-protein kinase/endoribonuclease IRE1
• NCBI Official Synonym Full Names: endoplasmic reticulum to nucleus signaling 1
• NCBI Official Symbol: ERN1
• NCBI Official Synonym Symbols: IRE1; IRE1P; IRE1a; hIRE1p
• NCBI Protein Information: serine/threonine-protein kinase/endoribonuclease IRE1
• UniProt Protein Name: Serine/threonine-protein kinase/endoribonuclease IRE1
• UniProt Gene Name: ERN1
• UniProt Synonym Gene Names: hIRE1p; IRE1a
• UniProt Entry Name: ERN1_HUMAN

NCBI Description

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]

Uniprot Description

IRE1: a ser/thr protein kinase that possess endonuclease activity. Important in altering gene expression as a response to endoplasmic reticulum based stress signals. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain, which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ubiquitously expressed. High levels observed in pancreatic tissue. Functionally connected with insulin biosynthesis in pancreatic beta cells. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of IRE1.

Protein type: Protein kinase, Other; Chaperone; Apoptosis; Endoplasmic reticulum; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Ribonuclease; Kinase, protein; Membrane protein, integral; Other group; IRE family

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane; nuclear inner membrane

Molecular Function: endoribonuclease activity; identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein homodimerization activity; magnesium ion binding; ADP binding; ATP binding

Biological Process: transcription, DNA-dependent; unfolded protein response; protein amino acid autophosphorylation; RNA splicing; positive regulation of RNA splicing; protein amino acid phosphorylation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of transcription, DNA-dependent; endothelial cell proliferation; UFP-specific transcription factor mRNA processing during unfolded protein response; mRNA catabolic process; cell cycle arrest; regulation of macroautophagy; mRNA cleavage