ERCC3 sirna

ERCC3 siRNA (Human)

Cat. Num. AAA8227782

• Gene Names: ERCC3; XPB; BTF2; TTD2; GTF2H; RAD25; TFIIH
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERCC3; ERCC3 siRNA (Human); XPB; XPBC; TFIIH basal transcription factor complex helicase XPB subunit; Basic transcription factor 2 89 kDa subunit; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH 89 kDa subunit; TFIIH p89; Xeroderma pigmentosum group B-complementing protein; ERCC3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ERCC3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 782

• Applicable Applications for ERCC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ERCC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERCC3 sirna

siRNA to inhibit ERCC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4557563
• NCBI GeneID: 2071
• NCBI Accession #: NP_000113.1
• NCBI GenBank Nucleotide #: NM_000122.1
• UniProt Accession #: P19447; Q53QM0
• Molecular Weight: 89,278 Da
• NCBI Official Full Name: TFIIH basal transcription factor complex helicase XPB subunit isoform a
• NCBI Official Synonym Full Names: excision repair cross-complementation group 3
• NCBI Official Symbol: ERCC3
• NCBI Official Synonym Symbols: XPB; BTF2; TTD2; GTF2H; RAD25; TFIIH
• NCBI Protein Information: TFIIH basal transcription factor complex helicase XPB subunit
• UniProt Protein Name: TFIIH basal transcription factor complex helicase XPB subunit
• Protein Family: Probable DNA repair helicase
• UniProt Gene Name: ERCC3
• UniProt Synonym Gene Names: XPB; XPBC; BTF2 p89; TFIIH 89 kDa subunit; TFIIH p89
• UniProt Entry Name: ERCC3_HUMAN

NCBI Description

This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

XPB: ATP-dependent 3'-5' DNA helicase, component of the core- TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with PUF60. Interacts with ATF7IP. Interacts with Epstein-Barr virus EBNA2. Belongs to the helicase family. RAD25/XPB subfamily.

Protein type: Helicase; DNA repair, damage; Transcription factor; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: nucleoplasm; holo TFIIH complex; nucleus

Molecular Function: protein C-terminus binding; DNA-dependent ATPase activity; GTP binding; ATPase activity; 3'-5' DNA helicase activity; dATP binding; protein N-terminus binding; peptide binding; transcription factor binding; protein kinase activity; ATP-dependent DNA helicase activity; RNA polymerase subunit kinase activity; protein binding; DNA binding; damaged DNA binding; ATP binding

Biological Process: transcription from RNA polymerase II promoter; DNA topological change; viral reproduction; apoptosis; positive regulation of viral transcription; positive regulation of apoptosis; protein amino acid phosphorylation; regulation of gene expression, epigenetic; protein localization; mRNA capping; UV protection; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; response to UV; transcription initiation from RNA polymerase II promoter; RNA elongation from RNA polymerase I promoter; hair cell differentiation; transcription from RNA polymerase I promoter; nucleotide-excision repair, DNA incision; termination of RNA polymerase I transcription; DNA repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; transcription initiation from RNA polymerase I promoter

Disease: Trichothiodystrophy 2, Photosensitive; Xeroderma Pigmentosum, Complementation Group B