EPN1 sirna

EPN1 siRNA (Human)

Cat. Num. AAA8209427

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EPN1; EPN1 siRNA (Human); Epsin-1; EH domain-binding mitotic phosphoprotein; EPS-15-interacting protein 1; EPN1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EPN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 662

• Applicable Applications for EPN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EPN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EPN1 sirna

siRNA to inhibit EPN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194272148
• NCBI GeneID: 29924
• NCBI Accession #: NP_001123543.1
• NCBI GenBank Nucleotide #: NM_001130071.1
• UniProt Accession #: Q9Y6I3; Q86ST3; Q9HA18
• Molecular Weight: 57,504 Da
• NCBI Official Full Name: epsin-1 isoform a
• NCBI Official Synonym Full Names: epsin 1
• NCBI Official Symbol: EPN1
• NCBI Protein Information: epsin-1
• UniProt Protein Name: Epsin-1
• UniProt Gene Name: EPN1
• UniProt Entry Name: EPN1_HUMAN

NCBI Description

The protein encoded by this gene binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

epsin 1: Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations. Regulates receptor-mediated endocytosis. Belongs to the epsin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: cytoplasm; plasma membrane; coated pit; nucleus

Molecular Function: lipid binding; transcription factor binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; Notch signaling pathway; in utero embryonic development; embryonic organ development; endocytosis; female pregnancy