EOGT sirna

EOGT siRNA (Human)

Cat. Num. AAA8235668

• Gene Names: EOGT; AOS4; AER61; EOGT1; C3orf64
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EOGT; EOGT siRNA (Human); AER61; C3orf64; EOGT1; EGF domain-specific O-linked N-acetylglucosamine transferase; Extracellular O-linked N-acetylglucosamine transferase; EOGT sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EOGT siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 527

• Applicable Applications for EOGT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EOGT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EOGT sirna

siRNA to inhibit EOGT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 522838254
• NCBI GeneID: 285203
• NCBI Accession #: NP_001265618.1
• NCBI GenBank Nucleotide #: NM_001278689.1
• UniProt Accession #: Q5NDL2; Q6MZY0; Q6P985; Q6ZTV0; A8K2U1; B4DFH5; L7X1M5
• Molecular Weight: 52,297 Da
• NCBI Official Full Name: EGF domain-specific O-linked N-acetylglucosamine transferase isoform a
• NCBI Official Synonym Full Names: EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase
• NCBI Official Symbol: EOGT
• NCBI Official Synonym Symbols: AOS4; AER61; EOGT1; C3orf64
• NCBI Protein Information: EGF domain-specific O-linked N-acetylglucosamine transferase
• UniProt Protein Name: EGF domain-specific O-linked N-acetylglucosamine transferase
• Protein Family: EGF domain-specific O-linked N-acetylglucosamine transferase
• UniProt Gene Name: EOGT
• UniProt Synonym Gene Names: AER61; C3orf64; EOGT1
• UniProt Entry Name: EOGT_HUMAN

NCBI Description

This gene encodes an enzyme that acts in the lumen of the endoplasmic reticulum to catalyze the transfer of N-acetylglucosamine to serine or threonine residues of extracellular-targeted proteins. This enzyme modifies proteins containing eukaryotic growth factor (EGF)-like domains, including the Notch receptor, thereby regulating developmental signalling. Mutations in this gene have been observed in individuals with Adams-Oliver syndrome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

AER61: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N- acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains. Belongs to the glycosyltransferase 61 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.1.255; Transferase

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: endoplasmic reticulum lumen

Molecular Function: protein N-acetylglucosaminyltransferase activity

Biological Process: protein amino acid O-linked glycosylation

Disease: Adams-oliver Syndrome 4