Rabbit PDCD4 Polyclonal Antibody | anti-PDCD4 antibody

PDCD4 Antibody

Cat. Num. AAA151051

• Gene Names: PDCD4; H731
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: PDCD4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PDCD4; Polyclonal Antibody; PDCD4 Antibody; H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; programmed cell death 4 (neoplastic transformation inhibitor); anti-PDCD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PDCD4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-PDCD4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: PDCD4 antibody can be used for detection of PDCD4 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PDCD4 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human PDCD4.
• Buffer: PDCD4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PDCD4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 μg/mL.)

Related Product Information for anti-PDCD4 antibody

PDCD4 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

NCBI and Uniprot Product Information

• NCBI GI #: 21735598
• NCBI GeneID: 27250
• NCBI Accession #: NP_663314
• NCBI GenBank Nucleotide #: NM_145341.3
• UniProt Accession #: Q53EL6; O15501; Q5VZS6; Q6PJI5; Q8TAR5; Q99834; B2RCV4; B5ME91
• Molecular Weight: 50,576 Da
• NCBI Official Full Name: programmed cell death protein 4 isoform 2
• NCBI Official Synonym Full Names: programmed cell death 4 (neoplastic transformation inhibitor)
• NCBI Official Symbol: PDCD4
• NCBI Official Synonym Symbols: H731
• NCBI Protein Information: programmed cell death protein 4; protein 197/15a; nuclear antigen H731; neoplastic transformation inhibitor protein
• UniProt Protein Name: Programmed cell death protein 4
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD4
• UniProt Synonym Gene Names: H731
• UniProt Entry Name: PDCD4_HUMAN

NCBI Description

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Uniprot Description

PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: negative regulation of JNK activity; apoptosis; cell aging; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle