ENOSF1 sirna

ENOSF1 siRNA (Human)

Cat. Num. AAA8210646

• Gene Names: ENOSF1; RTS; TYMSAS; HSRTSBETA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ENOSF1; ENOSF1 siRNA (Human); RTS; TYMSAS; Mitochondrial enolase superfamily member 1; Antisense RNA to thymidylate synthase; rTS; ENOSF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ENOSF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 361

• Applicable Applications for ENOSF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ENOSF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ENOSF1 sirna

siRNA to inhibit ENOSF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 186972148
• NCBI GeneID: 55556
• NCBI Accession #: NP_001119595.1
• NCBI GenBank Nucleotide #: NM_001126123.3
• UniProt Accession #: Q7L5Y1; Q15407; Q15594; Q15595; Q6ZS08; Q9HAS5; Q9HAS6; A6NMP3; A8K9R5; B3KSL6; B3KXE4; D3DUH0
• Molecular Weight: 24,284 Da
• NCBI Official Full Name: mitochondrial enolase superfamily member 1 isoform rTSalpha
• NCBI Official Synonym Full Names: enolase superfamily member 1
• NCBI Official Symbol: ENOSF1
• NCBI Official Synonym Symbols: RTS; TYMSAS; HSRTSBETA
• NCBI Protein Information: mitochondrial enolase superfamily member 1
• UniProt Protein Name: Mitochondrial enolase superfamily member 1
• Protein Family: Mitochondrial enolase superfamily
• UniProt Gene Name: ENOSF1
• UniProt Synonym Gene Names: RTS; TYMSAS; rTS
• UniProt Entry Name: ENOF1_HUMAN

NCBI Description

This gene was originally identified as a naturally occurring antisense transcript to the human thymidylate synthase gene. Alternate splice variants have been described, one of which (named rTSalpha) represents an alternate 3'UTR that is complementary to the 3'UTR and terminal intron of the thymidylate synthase (TS) RNA and down-regulates TS expression. Other transcript variants (rTSbeta and rTSgamma) do not overlap the TS locus. The function of this gene appears to be primarily to regulate expression of the TS locus both via the antisense transcript as well as through the encoded proteins. [provided by RefSeq, Mar 2010]

Uniprot Description

ENOSF1: May regulate thymidylate synthase activity. Belongs to the mandelate racemase/muconate lactonizing enzyme family. ENOSF1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; EC 4.2.1.68; Mitochondrial

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: mitochondrion

Molecular Function: magnesium ion binding; L-fuconate dehydratase activity; isomerase activity

Biological Process: cellular carbohydrate catabolic process; amino acid catabolic process