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EME1 sirna

EME1 siRNA (Human)

Gene Names
EME1; MMS4L; SLX2A
Reactivity
Human
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
EME1; EME1 siRNA (Human); MMS4; Crossover junction endonuclease EME1; MMS4 homolog; hMMS4; EME1 sirna
Ordering
For Research Use Only!
Host
Synthetic
Reactivity
Human
Specificity
EME1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
583
Applicable Applications for EME1 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EME1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for EME1 sirna
siRNA to inhibit EME1 expression using RNA interference

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,779 Da
NCBI Official Full Name
crossover junction endonuclease EME1 isoform 1
NCBI Official Synonym Full Names
essential meiotic structure-specific endonuclease 1
NCBI Official Symbol
EME1
NCBI Official Synonym Symbols
MMS4L; SLX2A
NCBI Protein Information
crossover junction endonuclease EME1
UniProt Protein Name
Crossover junction endonuclease EME1
UniProt Gene Name
EME1
UniProt Synonym Gene Names
MMS4; hMMS4
UniProt Entry Name
EME1_HUMAN

NCBI Description

This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]

Uniprot Description

EME1: Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. Belongs to the EME1/MMS4 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.22.-; Deoxyribonuclease; Nucleolus

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: cytoplasm; nucleolus; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; endonuclease activity

Biological Process: DNA repair; DNA recombination

Research Articles on EME1

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Product Notes

The EME1 eme1 (Catalog #AAA8214669) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The EME1 siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EME1 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the EME1 eme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EME1, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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