ELOVL6 sirna

ELOVL6 siRNA (Human)

Cat. Num. AAA8234580

• Gene Names: ELOVL6; FAE; LCE; FACE
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ELOVL6; ELOVL6 siRNA (Human); FACE; LCE; Elongation of very long chain fatty acids protein 6; 3-keto acyl-CoA synthase ELOVL6; ELOVL fatty acid elongase 6; ELOVL FA elongase 6; Fatty acid elongase 2; hELO2; Fatty acyl-CoA elongase; Long-chain fatty-acyl elongase; Very-long-chain 3-oxoacyl-CoA synthase 6; ELOVL6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ELOVL6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 265

• Applicable Applications for ELOVL6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ELOVL6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ELOVL6 sirna

siRNA to inhibit ELOVL6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 195539343
• NCBI GeneID: 79071
• NCBI Accession #: NP_001124193.1
• NCBI GenBank Nucleotide #: NM_001130721.1
• UniProt Accession #: Q9H5J4; Q4W5L0; Q8NCD1
• Molecular Weight: 31,376 Da
• NCBI Official Full Name: elongation of very long chain fatty acids protein 6
• NCBI Official Synonym Full Names: ELOVL fatty acid elongase 6
• NCBI Official Symbol: ELOVL6
• NCBI Official Synonym Symbols: FAE; LCE; FACE
• NCBI Protein Information: elongation of very long chain fatty acids protein 6
• UniProt Protein Name: Elongation of very long chain fatty acids protein 6
• UniProt Gene Name: ELOVL6
• UniProt Synonym Gene Names: FACE; LCE; ELOVL FA elongase 6; hELO2
• UniProt Entry Name: ELOV6_HUMAN

NCBI Description

Fatty acid elongases (EC 6.2.1.3), such as ELOVL6, use malonyl-CoA as a 2-carbon donor in the first and rate-limiting step of fatty acid elongation (Moon et al., 2001 [PubMed 11567032]).[supplied by OMIM, Mar 2008]

Uniprot Description

ELOVL6: Condensing enzyme that catalyzes the synthesis of saturated and monounsaturated fatty acids. Highest activity toward C16:0 acyl-CoAs. Belongs to the ELO family.

Protein type: EC 2.3.1.199; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; transferase activity, transferring groups other than amino-acyl groups

Biological Process: long-chain fatty acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid elongation, saturated fatty acid