EIF2D sirna

EIF2D siRNA (Human)

Cat. Num. AAA828368

• Gene Names: EIF2D; LGTN; HCA56
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF2D; EIF2D siRNA (Human); HCA56; LGTN; Eukaryotic translation initiation factor 2D; eIF2d; Hepatocellular carcinoma-associated antigen 56; Ligatin; EIF2D sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EIF2D siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 460

• Applicable Applications for EIF2D sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EIF2D gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF2D sirna

siRNA to inhibit EIF2D expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 319918871
• NCBI GeneID: 1939
• NCBI Accession #: NP_001188407.1
• NCBI GenBank Nucleotide #: NM_001201478.1
• UniProt Accession #: P41214; Q5SY40; Q8IXV3; Q96DG3; Q96TG7; Q9NR27; Q9NSN0; Q9NV18; Q9NZ21
• Molecular Weight: 51,013 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2D isoform 2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2D
• NCBI Official Symbol: EIF2D
• NCBI Official Synonym Symbols: LGTN; HCA56
• NCBI Protein Information: eukaryotic translation initiation factor 2D
• UniProt Protein Name: Eukaryotic translation initiation factor 2D
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF2D
• UniProt Synonym Gene Names: HCA56; LGTN; eIF2d
• UniProt Entry Name: EIF2D_HUMAN

NCBI Description

This gene encodes a translation initiation factor involved in the recruitment and delivery of aminoacyl-tRNAs to the P-site of the eukaryotic ribosome in a GTP-independent manner. This gene was previously referred to as ligatin, but is now known to localize to the cytoplasm and localize and function with translation factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Ligatin: Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P- site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Belongs to the eIF2D family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: translation initiation factor activity; receptor activity

Biological Process: intracellular protein transport; ribosome disassembly; formation of translation preinitiation complex