Human Laminin Alpha-4 ELISA Kit | LAMA4 elisa kit

Human Laminin Alpha-4 ELISA Kit

Cat. Num. AAA3804632

• Gene Names: LAMA4; LAMA3; CMD1JJ; LAMA4*-1
• Reactivity: Human
• Synonyms: Laminin Alpha-4; Human Laminin Alpha-4 ELISA Kit; LAMA4 elisa kit

• Reactivity: Human
• Sequence Length: 1823

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 2.5ng/ml-40ng/ml
• Sensitivity: 0.1 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMA4 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHuman Laminin alpha-4 in the sample, This Human Laminin alpha-4 Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHuman Laminin alpha-4 concentration. The concentration ofHuman Laminin alpha-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 380503843
• NCBI GeneID: 3910
• NCBI GenBank Nucleotide #: NP_001098676.2
• UniProt Accession #: Q16363
• NCBI Official Full Name: laminin subunit alpha-4 isoform 1
• NCBI Official Synonym Full Names: laminin subunit alpha 4
• NCBI Official Symbol: LAMA4
• NCBI Official Synonym Symbols: LAMA3; CMD1JJ; LAMA4*-1
• NCBI Protein Information: laminin subunit alpha-4
• UniProt Protein Name: Laminin subunit alpha-4
• Protein Family: Laminin
• UniProt Gene Name: LAMA4
• UniProt Entry Name: LAMA4_HUMAN

NCBI Description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

Uniprot Description

LAMA4: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: extracellular matrix; laminin-1 complex; extracellular region; basement membrane; basal lamina

Molecular Function: protein binding; extracellular matrix structural constituent; receptor binding

Biological Process: regulation of cell adhesion; extracellular matrix organization and biogenesis; regulation of embryonic development; cell adhesion; regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1jj