EHMT2 sirna

EHMT2 siRNA (Human)

Cat. Num. AAA8236953

• Gene Names: EHMT2; G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EHMT2; EHMT2 siRNA (Human); BAT8; C6orf30; G9A; KMT1C; NG36; Histone-lysine N-methyltransferase EHMT2; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Lysine N-methyltransferase 1C; Protein G9a; EHMT2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EHMT2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 202

• Applicable Applications for EHMT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EHMT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EHMT2 sirna

siRNA to inhibit EHMT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 574957264
• NCBI GeneID: 10919
• NCBI Accession #: NP_001276342.1
• NCBI GenBank Nucleotide #: NM_001289413.1
• UniProt Accession #: Q96KQ7; Q14349; Q5JP83; Q5JQ92; Q5JQA1; Q5JQG3; Q6PK06; Q96MH5; Q96QD0; Q9UQL8; Q9Y331; B0UZY2
• Molecular Weight: 19,864 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase EHMT2 isoform c
• NCBI Official Synonym Full Names: euchromatic histone-lysine N-methyltransferase 2
• NCBI Official Symbol: EHMT2
• NCBI Official Synonym Symbols: G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
• NCBI Protein Information: histone-lysine N-methyltransferase EHMT2
• UniProt Protein Name: Histone-lysine N-methyltransferase EHMT2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: EHMT2
• UniProt Synonym Gene Names: BAT8; C6orf30; G9A; KMT1C; NG36; H3-K9-HMTase 3
• UniProt Entry Name: EHMT2_HUMAN

NCBI Description

This gene is located in a gene cluster within the human major histocompatibility complex class III region on chromosome 6. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but recent publications show that there is only a single gene at this locus. The encoded protein is a histone methyltransferase, which may be involved in repression of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

EHMT2 iso4: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleus

Molecular Function: protein binding; zinc ion binding; p53 binding; protein-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; histone H3-K9 methylation; regulation of DNA replication