EFNA5 sirna

EFNA5 siRNA (Human)

Cat. Num. AAA8211289

• Gene Names: EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EFNA5; EFNA5 siRNA (Human); EPLG7; LERK7; Ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; LERK-7; EFNA5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EFNA5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 228

• Applicable Applications for EFNA5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EFNA5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EFNA5 sirna

siRNA to inhibit EFNA5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503487
• NCBI GeneID: 1946
• NCBI Accession #: NP_001953.1
• NCBI GenBank Nucleotide #: NM_001962.2
• UniProt Accession #: P52803
• Molecular Weight: 26,297 Da
• NCBI Official Full Name: ephrin-A5
• NCBI Official Synonym Full Names: ephrin-A5
• NCBI Official Symbol: EFNA5
• NCBI Official Synonym Symbols: AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
• NCBI Protein Information: ephrin-A5
• UniProt Protein Name: Ephrin-A5
• Protein Family: Ephrin
• UniProt Gene Name: EFNA5
• UniProt Synonym Gene Names: EPLG7; LERK7; LERK-7
• UniProt Entry Name: EFNA5_HUMAN

NCBI Description

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA5: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion. Belongs to the ephrin family.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: anchored to external side of plasma membrane; plasma membrane; extracellular region; caveola

Molecular Function: ephrin receptor binding; chemorepellent activity

Biological Process: axon guidance; nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell-cell adhesion; apoptosis; regulation of actin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; ephrin receptor signaling pathway; negative chemotaxis; retinal ganglion cell axon guidance