Human Ephrin A5 ELISA Kit | EFNA5 elisa kit

Human Ephrin A5 ELISA Kit

Cat. Num. AAA764950

• Gene Names: EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
• Reactivity: Human
• Synonyms: Ephrin A5; Human Ephrin A5 ELISA Kit; EFNA5/AL-1/EFL-5/LERK-7/RAGS/AF1/AL-1/EPH-related receptor tyrosine kinase ligand 7/EPLG7/GLC1M/LERK-7/LERK7EFL5/RAGS; EFNA5 elisa kit

• Reactivity: Human
• Sequence Length: 228

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EFNA5 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human EFNA5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EFNA5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EFNA5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EFNA5 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503487
• NCBI GeneID: 1946
• NCBI Accession #: NP_001953.1
• NCBI GenBank Nucleotide #: NM_001962.2
• Molecular Weight: 26,297 Da
• NCBI Official Full Name: ephrin-A5
• NCBI Official Synonym Full Names: ephrin-A5
• NCBI Official Symbol: EFNA5
• NCBI Official Synonym Symbols: AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
• NCBI Protein Information: ephrin-A5
• UniProt Protein Name: Ephrin-A5
• Protein Family: Ephrin
• UniProt Gene Name: EFNA5
• UniProt Synonym Gene Names: EPLG7; LERK7; LERK-7
• UniProt Entry Name: EFNA5_HUMAN

NCBI Description

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA5: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion. Belongs to the ephrin family.

Protein type: Ligand, receptor tyrosine kinase; Cell development/differentiation; Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: anchored to external side of plasma membrane; caveola; extracellular region; plasma membrane

Molecular Function: chemorepellent activity; ephrin receptor binding; neurotrophin TRKA receptor binding; neurotrophin TRKB receptor binding; neurotrophin TRKC receptor binding; transmembrane receptor protein tyrosine kinase activator activity

Biological Process: apoptosis; axon guidance; collateral sprouting; ephrin receptor signaling pathway; negative chemotaxis; nervous system development; positive regulation of collateral sprouting; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of synaptogenesis; regulation of actin cytoskeleton organization and biogenesis; regulation of cell-cell adhesion; regulation of focal adhesion formation; regulation of GTPase activity; retinal ganglion cell axon guidance; transmembrane receptor protein tyrosine kinase activation (dimerization)