EED sirna

EED siRNA (Human)

Cat. Num. AAA8203092

• Gene Names: EED; HEED; WAIT1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EED; EED siRNA (Human); Polycomb protein EED; hEED; WD protein associating with integrin cytoplasmic tails 1; WAIT-1; EED sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EED siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 466

• Applicable Applications for EED sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EED gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EED sirna

siRNA to inhibit EED expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 808175997
• NCBI GeneID: 8726
• NCBI Accession #: NP_001294936.1
• NCBI GenBank Nucleotide #: NM_001308007.1
• UniProt Accession #: O75530; O00149; Q6NTH2; Q7LDA5; Q7LDG8; Q86VV2; Q9UNY7; A8K7V5
• Molecular Weight: 45,524 Da
• NCBI Official Full Name: polycomb protein EED isoform c
• NCBI Official Synonym Full Names: embryonic ectoderm development
• NCBI Official Symbol: EED
• NCBI Official Synonym Symbols: HEED; WAIT1
• NCBI Protein Information: polycomb protein EED
• UniProt Protein Name: Polycomb protein EED
• Protein Family: Polycomb protein
• UniProt Gene Name: EED
• UniProt Synonym Gene Names: hEED; WAIT-1
• UniProt Entry Name: EED_HUMAN

NCBI Description

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EED: a member of the superfamily of Polycomb group (PcG) methyltransferase proteins with WD-40 repeats. PcG complexes mediate chromatin modifications that contribute to transcriptional silencing during development and carcinogenesis. A part of the Polycomb-repressive complex 2 (PRC2) along with EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. Interacts with HDAC, HDAC2, histone H1 and YY1. PRC2 trimethylates histone H3 on K9 and K27, transcriptionally repressing target gene including HOXC8, HOXA9, MYT1 and CDKN2A. Appears to be overexpressed in breast and colon cancers. Inhibitors of PRC2-mediated gene repression are being investigated as a cancer therapy. EED and EZH2 are also implicated in the silencing of the inactive X chromosome. Three human isoforms produced by alternative splicing or initiation have been described, and additional isoforms may be produced by alternative initiation.

Protein type: Methyltransferase

Chromosomal Location of Human Ortholog: 11q14.2-q22.3

Cellular Component: nucleoplasm; pronucleus; ESC/E(Z) complex; sex chromatin; cytoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; histone methyltransferase activity; protein binding; chromatin binding

Biological Process: histone methylation; genetic imprinting; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic