EDF1 sirna

EDF1 siRNA (Human)

Cat. Num. AAA8240215

• Gene Names: EDF1; MBF1; EDF-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EDF1; EDF1 siRNA (Human); Endothelial differentiation-related factor 1; EDF-1; Multiprotein-bridging factor 1; MBF1; EDF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EDF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 141

• Applicable Applications for EDF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EDF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EDF1 sirna

siRNA to inhibit EDF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 526253086
• NCBI GeneID: 8721
• NCBI Accession #: NP_001268226.1
• NCBI GenBank Nucleotide #: NM_001281297.1
• UniProt Accession #: O60869; Q5T5T2; Q9UIM1
• Molecular Weight: 15,636 Da
• NCBI Official Full Name: endothelial differentiation-related factor 1 isoform 3
• NCBI Official Synonym Full Names: endothelial differentiation-related factor 1
• NCBI Official Symbol: EDF1
• NCBI Official Synonym Symbols: MBF1; EDF-1
• NCBI Protein Information: endothelial differentiation-related factor 1
• UniProt Protein Name: Endothelial differentiation-related factor 1
• Protein Family: Endothelial differentiation-related factor
• UniProt Gene Name: EDF1
• UniProt Synonym Gene Names: EDF-1; MBF1
• UniProt Entry Name: EDF1_HUMAN

NCBI Description

This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

EDF1: Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nuclear receptor co-regulator; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: transcription factor TFIID complex; cytoplasm; nucleolus; intracellular; nucleus

Molecular Function: calmodulin binding; methyltransferase activity; protein binding; histone acetyltransferase activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity

Biological Process: endothelial cell differentiation; regulation of lipid metabolic process; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of DNA binding