Goat TBC1D4 / AS160 Polyclonal Antibody | anti-TBC1D4 antibody

Goat Polyclonal to Human TBC1D4 / AS160

Cat. Num. AAA242215

• Gene Names: TBC1D4; AS160; NIDDM5
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Orangutan, Monkey, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TBC1D4 / AS160; Polyclonal Antibody; Goat Polyclonal to Human TBC1D4 / AS160; Anti-TBC1D4 / AS160 Antibody (Internal) IHC-plus; TBC1D4; Akt substrate of 160 kDa; AS160; KIAA0603; TBC1 domain family; member 4; TBC1 domain family member 4; Human TBC1D4; anti-TBC1D4 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Orangutan, Monkey, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TBC1D4.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1290

• Applicable Applications for anti-TBC1D4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-DDPEKIEERKKSK from an internal region of human TBC1D4 (NP_055647.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Orangutan, Marmoset, Rabbit (92%); Panda, Bat (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: TBC1D4 / AS160 antibody was raised against synthetic peptide C-DDPEKIEERKKSK from an internal region of human TBC1D4 (NP_055647.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Orangutan, Marmoset, Rabbit (92%); Panda, Bat (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TBC1D4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

Western Blot (WB)

(Antibody (0.1 ug/ml) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 557786188
• NCBI GeneID: 9882
• NCBI Accession #: NP_001273587.1
• NCBI GenBank Nucleotide #: NM_001286658.1
• UniProt Accession #: O60343; Q5W0B9; Q68D14; A7E2X8; B4DU25; B4E235; B6ETN8; B6ETN9
• Molecular Weight: 60,095 Da
• NCBI Official Full Name: TBC1 domain family member 4 isoform 2
• NCBI Official Synonym Full Names: TBC1 domain family, member 4
• NCBI Official Symbol: TBC1D4
• NCBI Official Synonym Symbols: AS160; NIDDM5
• NCBI Protein Information: TBC1 domain family member 4; akt substrate of 160 kDa; Acrg embryonic lethality minimal region ortholog; TBC (Tre-2, BUB2, CDC16) domain-containing protein
• UniProt Protein Name: TBC1 domain family member 4
• Protein Family: TBC1 domain family
• UniProt Gene Name: TBC1D4
• UniProt Synonym Gene Names: AS160; KIAA0603; AS160
• UniProt Entry Name: TBCD4_HUMAN

NCBI Description

This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

AS160: a widely-expressed Rab GTPase-activating protein which apparently plays a role in mediating insulin action. Phosphorylated by the Ser/Thr protein kinase Akt in response to insulin. Is redistributed from low-density microsomes to the cytosol upon insulin treatment. Insulin stimulation of GLUT4 exocytosis is dependent on AS160. The translocation of GLUT4 from intracellular storage sites to the cell surface functions to stimulate insulin uptake by adipose and muscle tissue. Its expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects; its phosphorylation is impaired in skeletal muscle of type 2 diabetics. The protein is expressed in pancreatic Langerhans islets, including beta cells. Up-regulated in T cells from patients with atopic dermatitis. Two alternatively spliced isoforms have been described. Isoform 2, which promotes GLUT4 translocation at the plasma membrane, is the highest overexpressed in most tissues. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver or adipose tissue.

Protein type: GAPs; GAPs, Rab

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: cytoplasmic vesicle membrane; cytoplasm

Biological Process: vesicle-mediated transport; negative regulation of vesicle fusion; cellular response to insulin stimulus

Disease: Diabetes Mellitus, Noninsulin-dependent, 5