EDARADD sirna

EDARADD siRNA (Human)

Cat. Num. AAA827743

• Gene Names: EDARADD; ED3; EDA3; ECTD11A; ECTD11B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EDARADD; EDARADD siRNA (Human); Ectodysplasin-A receptor-associated adapter protein; EDAR-associated death domain protein; Protein crinkled homolog; EDARADD sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EDARADD siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 205

• Applicable Applications for EDARADD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EDARADD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EDARADD sirna

siRNA to inhibit EDARADD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18152769
• NCBI GeneID: 128178
• NCBI Accession #: NP_542776.1
• NCBI GenBank Nucleotide #: NM_080738.3
• UniProt Accession #: Q8WWZ3; Q5VYJ7; A2VCK5; A8K7B5; B1AL54; B9ZVW5
• Molecular Weight: 23,690 Da
• NCBI Official Full Name: ectodysplasin-A receptor-associated adapter protein isoform B
• NCBI Official Synonym Full Names: EDAR-associated death domain
• NCBI Official Symbol: EDARADD
• NCBI Official Synonym Symbols: ED3; EDA3; ECTD11A; ECTD11B
• NCBI Protein Information: ectodysplasin-A receptor-associated adapter protein
• UniProt Protein Name: Ectodysplasin-A receptor-associated adapter protein
• Protein Family: Ectodysplasin-A receptor-associated adapter protein
• UniProt Gene Name: EDARADD
• UniProt Entry Name: EDAD_HUMAN

NCBI Description

This gene was identified by its association with ectodermal dysplasia, a genetic disorder characterized by defective development of hair, teeth, and eccrine sweat glands. The protein encoded by this gene is a death domain-containing protein, and is found to interact with EDAR, a death domain receptor known to be required for the development of hair, teeth and other ectodermal derivatives. This protein and EDAR are coexpressed in epithelial cells during the formation of hair follicles and teeth. Through its interaction with EDAR, this protein acts as an adaptor, and links the receptor to downstream signaling pathways. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

EDARADD: Adapter protein that interacts with EDAR DEATH domain and couples the receptor to EDA signaling pathway during morphogenesis of ectodermal organs. Mediates the activation of NF- kappa-B. Defects in EDARADD are a cause of ectodermal dysplasia anhidrotic (EDA); also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q42.3

Cellular Component: cytoplasm

Biological Process: hair follicle development; cell differentiation; signal transduction; odontogenesis of dentine-containing teeth

Disease: Ectodermal Dysplasia 11a, Hypohidrotic/hair/tooth Type, Autosomal Dominant; Ectodermal Dysplasia 10a, Hypohidrotic/hair/nail Type, Autosomal Dominant; Ectodermal Dysplasia 10b, Hypohidrotic/hair/tooth Type, Autosomal Recessive; Ectodermal Dysplasia 11b, Hypohidrotic/hair/tooth Type, Autosomal Recessive