ECHS1 sirna

ECHS1 siRNA (Human)

Cat. Num. AAA8230914

• Gene Names: ECHS1; SCEH; ECHS1D
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ECHS1; ECHS1 siRNA (Human); Enoyl-CoA hydratase mitochondrial; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH; ECHS1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ECHS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 290

• Applicable Applications for ECHS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ECHS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ECHS1 sirna

siRNA to inhibit ECHS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194097323
• NCBI GeneID: 1892
• NCBI Accession #: NP_004083.3
• NCBI GenBank Nucleotide #: NM_004092.3
• UniProt Accession #: P30084; O00739; Q5VWY1; Q96H54
• Molecular Weight: 31,387 Da
• NCBI Official Full Name: enoyl-CoA hydratase, mitochondrial
• NCBI Official Synonym Full Names: enoyl CoA hydratase, short chain, 1, mitochondrial
• NCBI Official Symbol: ECHS1
• NCBI Official Synonym Symbols: SCEH; ECHS1D
• NCBI Protein Information: enoyl-CoA hydratase, mitochondrial
• UniProt Protein Name: Enoyl-CoA hydratase, mitochondrial
• Protein Family: Enoyl-CoA hydratase
• UniProt Gene Name: ECHS1
• UniProt Synonym Gene Names: SCEH
• UniProt Entry Name: ECHM_HUMAN

NCBI Description

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]

Uniprot Description

ECHS1: Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Belongs to the enoyl-CoA hydratase/isomerase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - lysine degradation; Lipid Metabolism - fatty acid elongation in mitochondria; Carbohydrate Metabolism - propanoate; Lyase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 4.2.1.17; Carbohydrate Metabolism - butanoate; Secondary Metabolites Metabolism - limonene and pinene degradation; Lipid Metabolism - fatty acid; Amino Acid Metabolism - tryptophan; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.2-q26.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein binding; enoyl-CoA hydratase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process

Disease: Mitochondrial Short-chain Enoyl-coa Hydratase 1 Deficiency