DTNA sirna

DTNA siRNA (Human)

Cat. Num. AAA8235776

• Gene Names: DTNA; DTN; DRP3; DTN-A; LVNC1; D18S892E
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DTNA; DTNA siRNA (Human); DRP3; Dystrobrevin alpha; DTN-A; Alpha-dystrobrevin; Dystrophin-related protein 3; DTNA sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DTNA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 371

• Applicable Applications for DTNA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DTNA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DTNA sirna

siRNA to inhibit DTNA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 189571587
• NCBI GeneID: 1837
• NCBI Accession #: NP_001121647.1
• NCBI GenBank Nucleotide #: NM_001128175.1
• UniProt Accession #: Q9Y4J8; O15332; O15333; O75697; A8K541; A8MSZ0; A8MUY4; B4DGS6; B4DIR0; B4DIU8; M0QYX6; M0R397
• Molecular Weight: 58,472 Da
• NCBI Official Full Name: dystrobrevin alpha isoform 9
• NCBI Official Synonym Full Names: dystrobrevin, alpha
• NCBI Official Symbol: DTNA
• NCBI Official Synonym Symbols: DTN; DRP3; DTN-A; LVNC1; D18S892E
• NCBI Protein Information: dystrobrevin alpha
• UniProt Protein Name: Dystrobrevin alpha
• Protein Family: Dystrobrevin
• UniProt Gene Name: DTNA
• UniProt Synonym Gene Names: DRP3; DTN-A
• UniProt Entry Name: DTNA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DTNA: May be involved in the formation and stability of synapses as well as being involved in the clustering of nicotinic acetylcholine receptors. Defects in DTNA are the cause of left ventricular non- compaction type 1 (LVNC1). Left ventricular non- compaction is due to an arrest of myocardial morphogenesis. The disorder is characterized by a hypertrophic left ventricle with deep trabeculations and with poor systolic function, with or without associated left ventricular dilation. In some cases, it is associated with other congenital heart anomalies such as ventricular septal defects, pulmonic stenosis and atrial septal defects. The right ventricle may also be affected. Belongs to the dystrophin family. Dystrobrevin subfamily. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18q12

Cellular Component: extrinsic to internal side of plasma membrane; protein complex; axon; cytoplasm; synapse; cell junction; sarcolemma

Molecular Function: protein binding; zinc ion binding; PDZ domain binding

Biological Process: synaptic transmission; striated muscle contraction; neuromuscular synaptic transmission; signal transduction

Disease: Left Ventricular Noncompaction 1