DPAGT1 sirna

DPAGT1 siRNA (Human)

Cat. Num. AAA8232400

• Gene Names: DPAGT1; GPT; ALG7; DGPT; G1PT; UAGT; UGAT; CDG1J; CMS13; DPAGT; CDG-Ij; CMSTA2; DPAGT2; D11S366
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DPAGT1; DPAGT1 siRNA (Human); DPAGT2; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase; GlcNAc-1-P transferase; G1PT; GPT; N-acetylglucosamine-1-phosphate transferase; DPAGT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DPAGT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 408

• Applicable Applications for DPAGT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DPAGT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DPAGT1 sirna

siRNA to inhibit DPAGT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 42794009
• NCBI GeneID: 1798
• NCBI Accession #: NP_001373.2
• NCBI GenBank Nucleotide #: NM_001382.3
• UniProt Accession #: Q9H3H5; O15216; Q86WV9; Q9BWE6
• Molecular Weight: 34,270 Da
• NCBI Official Full Name: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
• NCBI Official Synonym Full Names: dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
• NCBI Official Symbol: DPAGT1
• NCBI Official Synonym Symbols: GPT; ALG7; DGPT; G1PT; UAGT; UGAT; CDG1J; CMS13; DPAGT; CDG-Ij; CMSTA2; DPAGT2; D11S366
• NCBI Protein Information: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
• UniProt Protein Name: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
• Protein Family: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
• UniProt Gene Name: DPAGT1
• UniProt Synonym Gene Names: DPAGT2; G1PT; GPT
• UniProt Entry Name: GPT_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008]

Uniprot Description

DPAGT1: Catalyzes the initial step in the synthesis of dolichol- P-P-oligosaccharides. Defects in DPAGT1 are the cause of congenital disorder of glycosylation type 1J (CDG1J). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase 4 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 2.7.8.15; Transferase; Membrane protein, multi-pass; Endoplasmic reticulum; Glycan Metabolism - N-glycan biosynthesis

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity; transferase activity, transferring glycosyl groups; phospho-N-acetylmuramoyl-pentapeptide-transferase activity

Biological Process: polysaccharide biosynthetic process; UDP-N-acetylglucosamine metabolic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation; protein amino acid N-linked glycosylation via asparagine; dolichol biosynthetic process; post-translational protein modification; protein oligomerization

Disease: Congenital Disorder Of Glycosylation, Type Ij; Myasthenic Syndrome, Congenital, With Tubular Aggregates 2