DOCK3 sirna

DOCK3 siRNA (Human)

Cat. Num. AAA8207539

• Gene Names: DOCK3; PBP; MOCA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DOCK3; DOCK3 siRNA (Human); KIAA0299; MOCA; Dedicator of cytokinesis protein 3; Modifier of cell adhesion; Presenilin-binding protein; PBP; DOCK3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DOCK3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,030

• Applicable Applications for DOCK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DOCK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DOCK3 sirna

siRNA to inhibit DOCK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31415870
• NCBI GeneID: 1795
• NCBI Accession #: NP_004938.1
• NCBI GenBank Nucleotide #: NM_004947.4
• UniProt Accession #: Q8IZD9; O15017
• Molecular Weight: 233,103 Da
• NCBI Official Full Name: dedicator of cytokinesis protein 3
• NCBI Official Synonym Full Names: dedicator of cytokinesis 3
• NCBI Official Symbol: DOCK3
• NCBI Official Synonym Symbols: PBP; MOCA
• NCBI Protein Information: dedicator of cytokinesis protein 3
• UniProt Protein Name: Dedicator of cytokinesis protein 3
• Protein Family: Dedicator of cytokinesis protein
• UniProt Gene Name: DOCK3
• UniProt Synonym Gene Names: KIAA0299; MOCA; PBP
• UniProt Entry Name: DOCK3_HUMAN

NCBI Description

This gene is specifically expressed in the central nervous system (CNS). It encodes a member of the DOCK (dedicator of cytokinesis) family of guanine nucleotide exchange factors (GEFs). This protein, dedicator of cytokinesis 3 (DOCK3), is also known as modifier of cell adhesion (MOCA) and presenilin-binding protein (PBP). The DOCK3 and DOCK1, -2 and -4 share several conserved amino acids in their DHR-2 (DOCK homology region 2) domains that are required for GEF activity, and bind directly to WAVE proteins [Wiskott-Aldrich syndrome protein (WASP) family Verprolin-homologous proteins] via their DHR-1 domains. The DOCK3 induces axonal outgrowth in CNS by stimulating membrane recruitment of the WAVE complex and activating the small G protein Rac1. This gene is associated with an attention deficit hyperactivity disorder-like phenotype by a complex chromosomal rearrangement. [provided by RefSeq, Aug 2010]

Uniprot Description

DOCK3: Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Its interaction with presenilin proteins as well as its ability to stimulate Tau/MAPT phosphorylation suggest that it may be involved in Alzheimer disease. Ectopic expression in nerve cells decreases the secretion of beta-amyloid APBA1 protein and lowers the rate of cell-substratum adhesion, suggesting that it may affect the function of some small GTPase involved in the regulation of actin cytoskeleton or cell adhesion receptors. A chromosomal aberration involving DOCK3 has been found in a family with early-onset behavioral/developmental disorder with features of attention deficit-hyperactivity disorder and intellectual disability. Inversion inv(3)(p14:q21). The inversion disrupts DOCK3 and SLC9A9. Belongs to the DOCK family.

Protein type: GEFs; GEFs, misc.; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: cytoplasm

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; SH3 domain binding

Biological Process: small GTPase mediated signal transduction; positive regulation of GTPase activity