DLG1 sirna

DLG1 siRNA (Human)

Cat. Num. AAA8202934

• Gene Names: DLG1; hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLG1; DLG1 siRNA (Human); Disks large homolog 1; Synapse-associated protein 97; SAP-97; SAP97; hDlg; DLG1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DLG1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 904

• Applicable Applications for DLG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DLG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLG1 sirna

siRNA to inhibit DLG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148539628
• NCBI GeneID: 1739
• NCBI Accession #: NP_001091894.1
• NCBI GenBank Nucleotide #: NM_001098424.1
• UniProt Accession #: Q12959; Q12958; A5YKK7; B4DGU1; B4DGZ8; B7ZMM0; B9EIQ5; D3DXB8; D3DXB9; E7EWL7; E9PG21
• Molecular Weight: 88,485 Da
• NCBI Official Full Name: disks large homolog 1 isoform 1
• NCBI Official Synonym Full Names: discs, large homolog 1 (Drosophila)
• NCBI Official Symbol: DLG1
• NCBI Official Synonym Symbols: hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1
• NCBI Protein Information: disks large homolog 1
• UniProt Protein Name: Disks large homolog 1
• Protein Family: Disks large
• UniProt Gene Name: DLG1
• UniProt Synonym Gene Names: SAP-97; SAP97
• UniProt Entry Name: DLG1_HUMAN

NCBI Description

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known. [provided by RefSeq, Feb 2011]

Uniprot Description

DLG1: the human homologue of the Drosophila discs large tumor suppressor is an essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Interacts through its guanylate kinase-like domain with DLGAP1/2/3/4, and MAP1A. May interact with PTEN, HTR2A and LRFN1. Interacts through its PDZ domains with NMDAR2A, KV1.1, KV1.2, KV1.3, KV1.4, KV1.5, GLUR1, GPR124 and GPR125. Interacts with cytoskeleton-associated proteins EPB41 and EZR. Found in a complex with KV1.5 and CAV3. Found in a complex with APC and CTNNB1. Interacts with CDH1 through binding to PIK3R1. Forms multiprotein complexes with CASK, LIN7A, LIN7B, LIN7C, APBA1, and KCNJ12. Interacts through its guanylate kinase-like domain with KIF13B. Forms a tripartite complex composed of DLG1, MPP7 and LIN7A/C. May interact with TJAP1. Interacts with TOPK and the HTLV-1 viral Tax and HPV-18 E6 papillomavirus (HPV) oncoproteins. The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells. The PDZ domains may also mediate association to membranes by binding to EPB41 and GPR124 together with the L27 domain that binds CASK and DLG2. The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization. Processes such as wound healing and tissue maintenance dictate that cells maintain dynamic control of junction assembly and disassembly. Many pathologic processes such as cancer and invasion of host cells by microbes affect these pathways. The HPV-18 E6 oncogene sequesters INADL and its paralog MPDZ as well as DLG1 and targets them for degradation, contributing to loss of polarity. Seven human isoforms produced by alternative splicing have been described.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: Golgi apparatus; microtubule; basolateral plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft; postsynaptic membrane; perinuclear region of cytoplasm; cell projection membrane; cytoplasm; basal lamina; neuromuscular junction; lateral plasma membrane; endoplasmic reticulum membrane; internal side of plasma membrane; tight junction; lateral loop; endoplasmic reticulum; postsynaptic density; plasma membrane; ionotropic glutamate receptor complex; nucleus; cell junction; sarcolemma

Molecular Function: protein C-terminus binding; ionotropic glutamate receptor binding; guanylate kinase activity; protein binding; potassium channel regulator activity; mitogen-activated protein kinase kinase binding; cytoskeletal protein binding; protein complex scaffold; protein kinase binding; phosphoprotein phosphatase activity; phosphatase binding

Biological Process: lens development in camera-type eye; axon guidance; peristalsis; regulation of myelination; viral reproduction; formation of immunological synapse; T cell activation; positive regulation of potassium ion transport; establishment and/or maintenance of cell polarity; receptor clustering; smooth muscle development; synaptic transmission; cell-cell adhesion; negative regulation of T cell proliferation; T cell cytokine production; positive regulation of cell proliferation; hard palate development; negative regulation of mitotic cell cycle; cortical actin cytoskeleton organization and biogenesis; nucleotide phosphorylation; negative regulation of epithelial cell proliferation; nervous system development; negative regulation of protein kinase B signaling cascade; mitotic cell cycle checkpoint; actin filament organization; embryonic skeletal morphogenesis; establishment and/or maintenance of epithelial cell polarity; regulation of membrane potential; positive regulation of actin filament polymerization; dephosphorylation; amyloid precursor protein metabolic process; ureteric bud branching; endothelial cell proliferation; activation of protein kinase activity; lipid raft organization and biogenesis; reproductive structure development