DIAPH3 sirna

DIAPH3 siRNA (Human)

Cat. Num. AAA8201402

• Gene Names: DIAPH3; AN; DIA2; DRF3; AUNA1; NSDAN; diap3; mDia2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DIAPH3; DIAPH3 siRNA (Human); DIAP3; Protein diaphanous homolog 3; Diaphanous-related formin-3; DRF3; MDia2; DIAPH3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DIAPH3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1193

• Applicable Applications for DIAPH3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DIAPH3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DIAPH3 sirna

siRNA to inhibit DIAPH3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110225351
• NCBI GeneID: 81624
• NCBI Accession #: NP_001035982.1
• NCBI GenBank Nucleotide #: NM_001042517.1
• UniProt Accession #: Q9NSV4; Q18P99; Q18PA0; Q18PA1; Q2KPB6; Q3ZK23; Q5JTP8; Q5T2S7; Q5XKF6; A2A3B8; A2A3B9; A2A3C0
• Molecular Weight: 127,856 Da
• NCBI Official Full Name: protein diaphanous homolog 3 isoform a
• NCBI Official Synonym Full Names: diaphanous-related formin 3
• NCBI Official Symbol: DIAPH3
• NCBI Official Synonym Symbols: AN; DIA2; DRF3; AUNA1; NSDAN; diap3; mDia2
• NCBI Protein Information: protein diaphanous homolog 3
• UniProt Protein Name: Protein diaphanous homolog 3
• Protein Family: Protein diaphanous
• UniProt Gene Name: DIAPH3
• UniProt Synonym Gene Names: DIAP3; DRF3
• UniProt Entry Name: DIAP3_HUMAN

NCBI Description

This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Diaphanous-3: Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Defects in DIAPH3 are the cause of auditory neuropathy, autosomal dominant, type 1 (AUNA1). A form of sensorineural hearing loss with absent or severely abnormal auditory brainstem response, in the presence of normal cochlear outer hair cell function and normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. A disease- causing mutation in the conserved 5'-UTR leads to increased protein expression (PubMed:20624953). Belongs to the formin homology family. Diaphanous subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q21.2

Cellular Component: cytosol; nucleus

Molecular Function: Rho GTPase binding; actin binding

Biological Process: spermatogenesis; actin cytoskeleton organization and biogenesis

Disease: Auditory Neuropathy, Autosomal Dominant, 1