DHX36 sirna

DHX36 siRNA (Human)

Cat. Num. AAA8238643

• Gene Names: DHX36; G4R1; RHAU; DDX36; MLEL1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DHX36; DHX36 siRNA (Human); DDX36; KIAA1488; MLEL1; RHAU; ATP-dependent RNA helicase DHX36; DEAH box protein 36; G4-resolvase 1; G4R1; MLE-like protein 1; RNA helicase associated with AU-rich element ARE; DHX36 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DHX36 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 994

• Applicable Applications for DHX36 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DHX36 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DHX36 sirna

siRNA to inhibit DHX36 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 167830436
• NCBI GeneID: 170506
• NCBI Accession #: NP_001107869.1
• NCBI GenBank Nucleotide #: NM_001114397.1
• UniProt Accession #: Q9H2U1; Q70JU3; Q8IYE5; Q9P240; B2RB00
• Molecular Weight: 111,479 Da
• NCBI Official Full Name: ATP-dependent RNA helicase DHX36 isoform 2
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box polypeptide 36
• NCBI Official Symbol: DHX36
• NCBI Official Synonym Symbols: G4R1; RHAU; DDX36; MLEL1
• NCBI Protein Information: ATP-dependent RNA helicase DHX36
• UniProt Protein Name: ATP-dependent RNA helicase DHX36
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DHX36
• UniProt Synonym Gene Names: DDX36; KIAA1488; MLEL1; RHAU; G4R1
• UniProt Entry Name: DHX36_HUMAN

NCBI Description

This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DHX36: Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. Belongs to the DEAD box helicase family. DEAH subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase; RNA-binding; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: chromosome, telomeric region; cytoplasm; cytosol; nucleus

Molecular Function: G-quadruplex DNA binding; DNA-dependent ATPase activity; protein binding; histone deacetylase binding; double-stranded RNA binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing; ossification; response to exogenous dsRNA; transcription, DNA-dependent; response to virus; RNA secondary structure unwinding; positive regulation of telomere maintenance; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter