DHDH sirna

DHDH siRNA (Human)

Cat. Num. AAA8226719

• Gene Names: DHDH; 2DD; HUM2DD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DHDH; DHDH siRNA (Human); 2DD; Trans-1.2-dihydrobenzene-1.2-diol dehydrogenase; D-xylose 1-dehydrogenase; D-xylose-NADP dehydrogenase; Dimeric dihydrodiol dehydrogenase; Hum2DD; DHDH sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DHDH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 334

• Applicable Applications for DHDH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DHDH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DHDH sirna

siRNA to inhibit DHDH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7657212
• NCBI GeneID: 27294
• NCBI Accession #: NP_055290.1
• NCBI GenBank Nucleotide #: NM_014475.3
• UniProt Accession #: Q9UQ10
• Molecular Weight: 36,382 Da
• NCBI Official Full Name: trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• NCBI Official Synonym Full Names: dihydrodiol dehydrogenase (dimeric)
• NCBI Official Symbol: DHDH
• NCBI Official Synonym Symbols: 2DD; HUM2DD
• NCBI Protein Information: trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• UniProt Protein Name: Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• Protein Family: Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• UniProt Gene Name: DHDH
• UniProt Synonym Gene Names: 2DD
• UniProt Entry Name: DHDH_HUMAN

NCBI Description

This gene encodes an enzyme that belongs to the family of dihydrodiol dehydrogenases, which exist in multiple forms in mammalian tissues and are involved in the metabolism of xenobiotics and sugars. These enzymes catalyze the NADP1-linked oxidation of transdihydrodiols of aromatic hydrocarbons to corresponding catechols. This enzyme is a dimeric dihydrodiol dehydrogenase, and it differs from monomeric dihydrodiol dehydrogenases in its high substrate specificity for trans-dihydrodiols of aromatic hydrocarbons in the oxidative direction. [provided by RefSeq, Jul 2008]

Uniprot Description

DHDH: an enzyme that belongs to the family of dihydrodiol dehydrogenases, which exist in multiple forms in mammalian tissues and are involved in the metabolism of xenobiotics and sugars. These enzymes catalyze the NADP1-linked oxidation of transdihydrodiols of aromatic hydrocarbons to corresponding catechols. This enzyme is a dimeric dihydrodiol dehydrogenase, and it differs from monomeric dihydrodiol dehydrogenases in its high substrate specificity for trans-dihydrodiols of aromatic hydrocarbons in the oxidative direction. [provided by RefSeq, Jul 2008]

Protein type: Oxidoreductase; EC 1.1.1.179; EC 1.3.1.20; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 19q13.3

Molecular Function: NAD(P) transhydrogenase activity; electron carrier activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; D-xylose 1-dehydrogenase (NADP+) activity

Biological Process: carbohydrate metabolic process; D-xylose catabolic process