CYP2C18 sirna

CYP2C18 siRNA (Human)

Cat. Num. AAA827442

• Gene Names: CYP2C18; CPCI; CYP2C; CYP2C17; P450IIC17; P450-6B/29C
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CYP2C18; CYP2C18 siRNA (Human); Cytochrome P450 2C18; CYPIIC18; Cytochrome P450-6b/29c; CYP2C18 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CYP2C18 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 490

• Applicable Applications for CYP2C18 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CYP2C18 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CYP2C18 sirna

siRNA to inhibit CYP2C18 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13699816
• NCBI GeneID: 1562
• NCBI Accession #: NP_000763.1
• NCBI GenBank Nucleotide #: NM_000772.2
• UniProt Accession #: P33260; Q16703; Q16751; Q4VAT5; Q6GRG1; B2R8K2
• Molecular Weight: 48,805 Da
• NCBI Official Full Name: cytochrome P450 2C18 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily C, polypeptide 18
• NCBI Official Symbol: CYP2C18
• NCBI Official Synonym Symbols: CPCI; CYP2C; CYP2C17; P450IIC17; P450-6B/29C
• NCBI Protein Information: cytochrome P450 2C18
• UniProt Protein Name: Cytochrome P450 2C18
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2C18
• UniProt Entry Name: CP2CI_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum but its specific substrate has not yet been determined. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. An additional gene, CYP2C17, was once thought to exist; however, CYP2C17 is now considered an artefact based on a chimera of CYP2C18 and CYP2C19. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2C18: a member of the cytochrome P450 superfamily of enzymes. Cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. Participates in the oxidation of a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Localizes to the endoplasmic reticulum but its specific substrate has not yet been determined. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.

Protein type: Lipid Metabolism - linoleic acid; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Lipid Metabolism - arachidonic acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding; monooxygenase activity

Biological Process: xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway