CYLC2 sirna
CYLC2 siRNA (Human)
Reactivity
Human
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
CYLC2; CYLC2 siRNA (Human); CYL2; Cylicin-2; Cylicin II; Multiple-band polypeptide II; CYLC2 sirna
Host
Synthetic
Reactivity
Human
Specificity
CYLC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
348
Applicable Applications for CYLC2 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CYLC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for CYLC2 sirna
siRNA to inhibit CYLC2 expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,079 Da
NCBI Official Full Name
cylicin-2
NCBI Official Synonym Full Names
cylicin, basic protein of sperm head cytoskeleton 2
NCBI Official Symbol
CYLC2
NCBI Protein Information
cylicin-2
UniProt Protein Name
Cylicin-2
Protein Family
UniProt Gene Name
CYLC2
UniProt Synonym Gene Names
CYL2
UniProt Entry Name
CYLC2_HUMAN
NCBI Description
Cylicin II (CYCL2) is specifically expressed in testis and is part of the cytoskeletal calyx of mammalian sperm heads. Cylicin II may play a role in the morphogenesis of the sperm head. [provided by RefSeq, Jul 2008]
Uniprot Description
CYLC2: Possible architectural role during spermatogenesis. May be involved in spermatid differentiation.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9q31.1
Cellular Component: nucleus
Molecular Function: structural constituent of cytoskeleton
Biological Process: multicellular organismal development; spermatogenesis; cell differentiation
Research Articles on CYLC2
Similar Products
Product Notes
The CYLC2 cylc2 (Catalog #AAA8200313) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The CYLC2 siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYLC2 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the CYLC2 cylc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYLC2, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
