CTSG sirna

CTSG siRNA (Human)

Cat. Num. AAA8224431

• Gene Names: CTSG; CG; CATG
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTSG; CTSG siRNA (Human); Cathepsin G; CG; CTSG sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CTSG siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 255

• Applicable Applications for CTSG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CTSG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTSG sirna

siRNA to inhibit CTSG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503149
• NCBI GeneID: 1511
• NCBI Accession #: NP_001902.1
• NCBI GenBank Nucleotide #: NM_001911.2
• UniProt Accession #: P08311; Q6IBJ6; Q9UCA5; Q9UCU6
• Molecular Weight: 28,837 Da
• NCBI Official Full Name: cathepsin G preproprotein
• NCBI Official Synonym Full Names: cathepsin G
• NCBI Official Symbol: CTSG
• NCBI Official Synonym Symbols: CG; CATG
• NCBI Protein Information: cathepsin G
• UniProt Protein Name: Cathepsin G
• Protein Family: Cathepsin
• UniProt Gene Name: CTSG
• UniProt Synonym Gene Names: CG
• UniProt Entry Name: CATG_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family.

Protein type: Motility/polarity/chemotaxis; Protease; EC 3.4.21.20

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus; secretory granule

Molecular Function: peptidase activity; heparin binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; positive regulation of immune response; extracellular matrix organization and biogenesis; cellular protein metabolic process; immune response; response to lipopolysaccharide; protein processing; angiotensin maturation; proteolysis; defense response to fungus