CTDP1 sirna

CTDP1 siRNA (Human)

Cat. Num. AAA8238213

• Gene Names: CTDP1; FCP1; CCFDN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTDP1; CTDP1 siRNA (Human); FCP1; RNA polymerase II subunit A C-terminal domain phosphatase; TFIIF-associating CTD phosphatase; CTDP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CTDP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 842

• Applicable Applications for CTDP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CTDP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTDP1 sirna

siRNA to inhibit CTDP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 321267522
• NCBI GeneID: 9150
• NCBI Accession #: NP_001189433.1
• NCBI GenBank Nucleotide #: NM_001202504.1
• UniProt Accession #: Q9Y5B0; Q7Z644; Q96BZ1; Q9Y6F5; A8MY97
• Molecular Weight: 93,485 Da
• NCBI Official Full Name: RNA polymerase II subunit A C-terminal domain phosphatase isoform 3
• NCBI Official Synonym Full Names: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1
• NCBI Official Symbol: CTDP1
• NCBI Official Synonym Symbols: FCP1; CCFDN
• NCBI Protein Information: RNA polymerase II subunit A C-terminal domain phosphatase
• UniProt Protein Name: RNA polymerase II subunit A C-terminal domain phosphatase
• UniProt Gene Name: CTDP1
• UniProt Synonym Gene Names: FCP1
• UniProt Entry Name: CTDP1_HUMAN

NCBI Description

This gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in this gene are associated with congenital cataracts, facial dysmorphism and neuropathy syndrome (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

CTDP1: a phospho-serine/threonine protein phosphatase which interacts with the carboxy-terminus of transcription initiation factor TFIIF-alpha, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. Its phosphorylation increases binding to TFIIF-alpha, affecting transcription elongation. Alternative splicing results in two different isoforms.

Protein type: Motility/polarity/chemotaxis; Transcription initiation complex; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; spindle; midbody; spindle midzone; actin cytoskeleton

Molecular Function: DNA-directed RNA polymerase activity; CTD phosphatase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; cell division; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; protein amino acid dephosphorylation; exit from mitosis

Disease: Congenital Cataracts, Facial Dysmorphism, And Neuropathy